酸性氧化电位水对铜绿假单胞菌的杀菌机制研究

发布时间：2018-06-06 00:18

本文选题：酸性氧化电位水 + 戊二醛　；参考：《中国感染控制杂志》2017年01期

【摘要】：目的探讨酸性氧化电位水(EOW)对铜绿假单胞菌的杀菌机制。方法以铜绿假单胞菌作为研究对象,从蛋白质渗漏,核酸和细胞膜钙离子通透性等方面对EOW的杀菌机制进行研究,同时以2%戊二醛作为阳性对照组,以生理盐水(NS)作为阴性对照组。结果以EOW、2%戊二醛对铜绿假单胞菌作用30 s杀灭率99.99%,作用60 s杀灭率可达100.00%。EOW、NS、2%戊二醛作用铜绿假单胞菌60 s后,经BCA法检测蛋白渗漏,分别为(96.00±7.42)、(94.15±7.49)、(216.97±10.35)μg/mL,总体比较,差异有统计学意义(F=613.20,P0.01),2%戊二醛组高于EOW组、NS组;蛋白渗漏量不随作用时间增加而改变(均P0.05)。随机多态性扩增DNA电泳图谱显示,EOW组与NS组均在500~1 000 kb间显示亮度较高的密集条带,2%戊二醛组无扩增条带。EOW及2%戊二醛作用后钙离子荧光强度均低于NS组。结论 EOW可能的杀菌机制是使铜绿假单胞菌细胞膜通透性受到一定程度的损伤,致使Ca2+泄漏,但未能造成蛋白质泄漏,对其核酸损伤作用不明显,DNA可能并非EOW杀菌靶点。
[Abstract]:Objective to study the bactericidal mechanism of EOW against Pseudomonas aeruginosa. Methods using Pseudomonas aeruginosa as the research object, the bactericidal mechanism of EOW was studied from the aspects of protein leakage, nucleic acid and membrane calcium permeability, and 2% glutaraldehyde was used as the positive control group. NS was used as negative control group. Results the killing rate of Pseudomonas aeruginosa exposed to EOW 2% glutaraldehyde for 30 s was 99.99, and the killing rate was 100.00.EOWN 2% Glutaraldehyde for 60 seconds. The protein leakage was detected by BCA method, which was 96.00 卤7.4224.15 卤7.497.49 渭 g 路mL ~ (-1) 渭 g 路mL ~ (-1), respectively. The difference was statistically significant (P < 0.01), but the protein leakage did not change with the increase of the time of action (all P 0.05), and the content of protein leakage was higher in the group of 2% glutaraldehyde than that in the group of EOW (P < 0.05). Random polymorphic DNA electrophoresis showed that the fluorescence intensity of calcium ion in EOW group and NS group was lower than that in NS group after the treatment of 2% glutaraldehyde and 2% glutaraldehyde. Conclusion the possible bactericidal mechanism of EOW is that the membrane permeability of Pseudomonas aeruginosa is damaged to a certain extent, which results in the leakage of Ca2, but does not cause the leakage of protein.
【作者单位】：第四军医大学西京医院;第四军医大学西京皮肤医院;
【分类号】：R187

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