钉螺压碎器批量检测钉螺应用效果

发布时间：2018-07-25 18:23

【摘要】：目的评价钉螺压碎器在批量检测血吸虫感染性钉螺中的应用效果。方法制作钉螺压碎器和逸蚴三角瓶,将未感染血吸虫的钉螺按50、100、200、300只分成4组,分别装入逸蚴三角瓶中,每组10瓶;从每组中随机选3瓶,将实验室人工培养的血吸虫感染性钉螺12只分别投入各瓶,每瓶1只;将装有感染性钉螺的逸蚴瓶放回各组统一编号。用钉螺压碎器压碎钉螺,记录各组碎螺率、施压次数与时间;待钉螺全部被压碎后,加水并取水膜至显微镜下观察,记录尾蚴数量并计算各组尾蚴检出率。现场采集钉螺,用压碎法和钉螺压碎器改良压碎逸蚴法(压逸法)进行检测,计算二者的符合率。结果采用钉螺压碎器压碎50、100、200、300只钉螺,各组碎螺率均为100%,每组平均施压次数分别为15.70、23.20、32.20、39.20次,平均施压时间分别为1.01、1.70、2.00、3.00 min,施压次数、时间与批量钉螺只数呈正相关(r=0.68、0.73,P均0.01);各组尾蚴检出率均为100%。现场应用中,压碎法检测钉螺109只,钉螺压碎器压逸法检测620只,均未发现感染性钉螺,两种方法符合率为100%。结论钉螺压碎器操作简单、省力,具有现场推广应用价值。
[Abstract]:Objective to evaluate the effect of Oncomelania Oncomelania Snail crusher in the mass detection of schistosomiasis infected Oncomelania snails. Methods the Oncomelania snails and the flask triangulation were made. The Oncomelania snails without schistosomiasis were divided into 4 groups, and 10 bottles in each group were loaded in each group, and 3 bottles were selected from each group to cultivate Schistosoma japonicum artificially in the laboratory. 12 infective Oncomelania Snail were put into each bottle, each bottle was 1. The infective Oncomelania flask was put back in each group. The snails were crushed by the Oncomelania snails, and the number of broken snails, the times and time of pressure were recorded. After all the snails were crushed, water was added to the microscopes to observe the number of cercariae and to calculate the detection of cercaria in each group. On the spot, the Oncomelania snails were collected by the crushing method and the amelioration of the Oncomelania snails crushing apparatus (Ya Yifa). The coincidence rate of the two were calculated. The results were that 50100200300 Oncomelania snails were crushed by the Oncomelania snails crushing device. The rate of broken snails in each group was 100%, and the average pressure times of each group were 15.70,23.20,32.20,39.20 times, and the average pressure time was 1.01,1, respectively. .70,2.00,3.00 min, the times of pressure and time were positively correlated with the number of Oncomelania hupensis (r=0.68,0.73, P 0.01), and the detection rate of cercariae in all groups was 100%. field application, 109 of Oncomelania snails were detected by crushing method, 620 were detected by Oncomelania snails crushing method, and none of infectious Oncomelania snails were detected, and the two methods of conformation of Oncomelania snails were simple, It is effective and has the value of application in the field.
【作者单位】：湖北省荆州市疾病预防控制中心血吸虫病预防控制所;中国疾病预防控制中心;
【基金】：中国疾病预防控制中心委托项目(201036-W-056)
【分类号】：R532.21;R184

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