义乌市职业暴露人群血清学和环境高致病性禽流感监测分析

发布时间：2018-07-26 12:33

【摘要】：目的了解高致病性H5N1禽流感病毒在浙江省义乌市职业暴露人群中的感染变化状况及其在环境中的分布情况。方法马血球血凝抑制试验(HI)检测高致病性H5N1禽流感病毒抗体,实时荧光定量PCR检测A型流感病毒。结果在105份职业暴露人群血清标本中检出H5N1抗体阳性标本12份,总检出率为11.43%。男、女性别间H5N1抗体阳性率差异无统计学意义(χ2=0.064,P0.05);家禽规模养殖场(户)和家禽散养户两类职业暴露人群间H5N1抗体阳性率差异无统计学意义(χ2=1.304,P0.05);226份环境标本中检出H9亚型禽流感病毒1份,未检出H5亚型。结论义乌市职业暴露人群中存在高致病性H5N1禽流感病毒隐性感染的情况或有个别轻症病例的可能,环境中存在禽流感病毒的污染,未发现高致病性H5N1禽流感病毒污染的证据,应加大监测力度。
[Abstract]:Objective to investigate the infection and distribution of highly pathogenic H5N1 avian influenza virus in occupational exposed population in Yiwu City, Zhejiang Province. Methods the antibody to highly pathogenic H5N1 avian influenza virus was detected by horse hemagglutination inhibition test (HI), and the influenza A virus was detected by real-time fluorescence quantitative PCR. Results among 105 serum samples of occupational exposed population, 12 were positive for H5N1 antibody, the total detection rate was 11.43. Male, There was no significant difference in the positive rate of H5N1 antibody among women (蠂 2 / 0.064), and there was no significant difference in the positive rate of H5N1 antibody between the exposed population of poultry farm (household) and poultry family (蠂 21.304). The H9 subtype was detected in 226 environmental samples. 1 copy of avian influenza virus, No H5 subtype was detected. Conclusion there is a recessive infection of highly pathogenic H5N1 avian influenza virus in occupational exposed population in Yiwu city, or the possibility of individual mild disease cases, and there is no evidence of highly pathogenic H5N1 avian influenza virus contamination in the environment. Monitoring should be strengthened.
【作者单位】：义乌市疾病预防控制中心;
【分类号】：R181.8

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