新型等离子体射流对粪肠球菌的灭菌实验研究
发布时间：2018-08-02 09:09
【摘要】：目的:本研究旨在探索一种全新的、有效的根管消毒方法,提高根管消毒的效能,从而提高根管治疗术的成功率。 方法:本研究采用一种新型纳秒脉冲驱动的大气压非平衡等离子体射流分别作用于粪肠球菌(Enterococcus faecalis,E. faecalis)标准株培养皿、E. faecalis硝酸纤维素生物膜模型以及离体牙根管E. faecalis感染模型。该新型等离子体射流在各物理参数恒定情况下,分别以不同时间、不同环境及不同形式作用于培养皿、生物膜模型和离体牙感染根管模型,对E. faecalis的灭菌效果进行了观察和测定,并分析了该等离子体射流灭菌效果的影响因素,还通过透射电镜观察处理后细菌形态的变化分析其灭菌机理。同时对经Micro-CT扫描观察到的各离体牙的根管形态与灭菌效果之间的关系进行了分析。 结果:本研究结果显示此新型大气压非平衡等离子体射流对体外培养的E. faecalis有良好的灭菌效果,并对E. faecalis生物膜模型及离体牙感染E. faecalis模型有一定的消毒灭菌作用。但这些灭菌效果与等离子体射流作用时间、作用环境以及感染根管的形态密切相关。 结论:此新型大气压非平衡等离子体射流可以作为根管消毒的辅助方法,但其灭菌作用的可靠性、有效性、安全性及其作用机理仍需进一步实验研究证实。
[Abstract]:Objective: to explore a new and effective method of root canal disinfection to improve the efficacy of root canal disinfection and to improve the success rate of root canal therapy. Methods: in this study, a novel nanosecond pulse-driven atmospheric pressure non-equilibrium plasma jet was applied to Enterococcus faecalis E. faecalis standard strain, E. faecalis nitrate cellulose biofilm model and in vitro root canal E. faecalis infection model. The new plasma jet acted on petri dish, biofilm model and tooth infected root canal model in different time, environment and form under constant physical parameters. The sterilizing effect of E. faecalis was observed and measured, and the influencing factors of the effect of plasma jet sterilization were analyzed. The sterilizing mechanism was also analyzed by observing the morphological changes of bacteria after treatment with transmission electron microscope. At the same time, the relationship between the root canal morphology and sterilizing effect of the isolated teeth observed by Micro-CT scan was analyzed. Results: the results showed that the new atmospheric pressure non-equilibrium plasma jet had a good sterilizing effect on E. faecalis cultured in vitro, and had a certain disinfection and sterilization effect on E. faecalis biofilm model and the model of isolated tooth infection E. faecalis. However, these sterilization effects are closely related to the time of plasma jet action, the environment of action and the morphology of infected root canal. Conclusion: this new type of atmospheric pressure non-equilibrium plasma jet can be used as an auxiliary method for root canal disinfection, but the reliability, effectiveness, safety and mechanism of its sterilization need further experimental study.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R187
本文编号：2158947
[Abstract]:Objective: to explore a new and effective method of root canal disinfection to improve the efficacy of root canal disinfection and to improve the success rate of root canal therapy. Methods: in this study, a novel nanosecond pulse-driven atmospheric pressure non-equilibrium plasma jet was applied to Enterococcus faecalis E. faecalis standard strain, E. faecalis nitrate cellulose biofilm model and in vitro root canal E. faecalis infection model. The new plasma jet acted on petri dish, biofilm model and tooth infected root canal model in different time, environment and form under constant physical parameters. The sterilizing effect of E. faecalis was observed and measured, and the influencing factors of the effect of plasma jet sterilization were analyzed. The sterilizing mechanism was also analyzed by observing the morphological changes of bacteria after treatment with transmission electron microscope. At the same time, the relationship between the root canal morphology and sterilizing effect of the isolated teeth observed by Micro-CT scan was analyzed. Results: the results showed that the new atmospheric pressure non-equilibrium plasma jet had a good sterilizing effect on E. faecalis cultured in vitro, and had a certain disinfection and sterilization effect on E. faecalis biofilm model and the model of isolated tooth infection E. faecalis. However, these sterilization effects are closely related to the time of plasma jet action, the environment of action and the morphology of infected root canal. Conclusion: this new type of atmospheric pressure non-equilibrium plasma jet can be used as an auxiliary method for root canal disinfection, but the reliability, effectiveness, safety and mechanism of its sterilization need further experimental study.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R187
【参考文献】
相关期刊论文 前1条
1 陈浩,梁沛,胡斌,赵丽,孙大海,王小如;电感耦合等离子体原子发射光谱/质谱法在中药微量元素及形态分析中的应用[J];光谱学与光谱分析;2002年06期
，本文编号：2158947
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