460nm可见光杀灭浮游状态和生物膜内大肠埃希菌的实验研究

发布时间：2019-01-19 10:50

【摘要】：目的探讨460 nm可见光(蓝光)对浮游状态和生物膜内大肠埃希菌的作用。方法自烧伤病房采集大肠埃希菌标本,经细菌鉴定仪鉴定;分别采用不同能量的460 nm可见光照射浮游状态大肠埃希菌,分别采用平板划线法、活/死菌染色法评价460 nm可见光的杀菌效果。制作大肠埃希菌生物膜模型,FM 1-43及结晶紫鉴定后给予240 J/cm2和360 J/cm2的460 nm可见光照射,活/死菌染色激光共聚焦显微镜观察生物膜内细菌的存活情况。以未经照射处理的标本作为对照。结果大肠埃希菌标本经60 J/cm2及以上剂量的460 nm可见光照射后,与对照组比较,平板划线后的菌落数明显减少,并呈剂量效应关系;240 J/cm2和360 J/cm2的460 nm可见光照射后,生物膜内各层细菌的死亡率均显著高于对照组。结论 460 nm可见光具有高效杀灭浮游状态和生物膜内大肠埃希菌的作用。
[Abstract]:Objective to investigate the effects of nm visible light (blue light) on planktonic state and biofilm Escherichia coli. Methods the specimens of Escherichia coli were collected from burn ward and identified by bacteria identification instrument. Escherichia coli was irradiated with the visible light of 460 nm with different energy. The bactericidal efficacy of the visible light of 460 nm was evaluated by plate marking method and living / dead bacteria staining method. A biofilm model of Escherichia coli was made. FM 1-43 and crystal violet were identified and irradiated with the visible light of 240 J/cm2 and 360 J/cm2 for 460 nm. The survival of bacteria in the biofilm was observed by laser confocal microscopy with live / dead bacteria staining. Unirradiated specimens were used as controls. Results the colony number of Escherichia coli specimens after 60 J/cm2 and above dose of 460 nm visible light irradiation was significantly decreased compared with the control group, and showed a dose-dependent relationship. The mortality of bacteria in each layer of biofilm was significantly higher than that in control group after exposure to the visible light of 240 J/cm2 and 360 J/cm2 for 460 nm. Conclusion the visible light of 460 nm can effectively kill zooplankton and Escherichia coli in biofilm.
【作者单位】：上海交通大学医学院附属第三人民医院烧伤整形科;
【基金】：国家自然科学基金(81272113) 上海交通大学科技基金项目(12XJ30062) 上海交通大学“医工(理)交叉研究基金”(yg2011zd04)~~
【分类号】：R187.4

【相似文献】