亚致死剂量球形芽孢杆菌对按蚊传疟能力的影响

发布时间：2019-03-18 18:12

【摘要】：目的探讨亚致死剂量球形芽孢杆菌(Bacillus sphaericus,Bs)对斯氏按蚊传疟能力的影响。方法利用亚致死剂量Bs 2 362株处理斯氏按蚊Ⅳ龄幼虫,收集存活按蚊并常规饲养。正常对照组按蚊Ⅳ龄幼虫不进行Bs处理,常规饲养。对3~5 d龄的斯氏按蚊成蚊进行约氏疟原虫攻击感染,并于按蚊吸血后第9天解剖蚊胃,观察卵囊的发育情况,比较分析Bs处理对斯氏按蚊传疟能力的影响。结果正常对照组和Bs处理组按蚊对疟原虫的感染率分别为92.1%和97.6%,Bs处理组感染率高于正常对照组,差异有统计学意义(P0.05)。Sigma 3.5统计软件分析显示,正常对照组和Bs处理组斯氏按蚊对疟原虫的感染度分别为(56.69±69.29)和(84.19±75.64),Bs处理组按蚊卵囊数量显著高于正常对照组,差异有统计学意义(P0.05)。结论亚致死剂量Bs 2362株处理显著增加约氏疟原虫对斯氏按蚊的感染率和感染度,提高了按蚊的传疟能力。
[Abstract]:Objective to investigate the effect of sublethal dose Bacillus sphaeroides (Bacillus sphaericus,Bs) on the ability of Anopheles stephensi to transmit malaria. Methods the fourth instar larvae of Anopheles stephensi were treated with sublethal dose of Bs 2362 strains, and the surviving Anopheles stephensi larvae were collected and fed routinely. The fourth instar larvae of Anopheles mosquitoes in normal control group were fed without Bs. The adult Anopheles stephensi (Anopheles stephensi) was infected with Plasmodium yoelii attack, and the stomach of Anopheles stephensi was dissected on the 9th day after blood feeding. The development of oocysts was observed and the effects of Bs treatment on the ability of malaria transmission were compared and analyzed in Anopheles stephensi. Results the infection rate of Anopheles mosquitoes to Plasmodium was 92.1% and 97.6% in the control group and Bs treatment group, respectively. The infection rate in the Bs treatment group was higher than that in the normal control group, the difference was statistically significant (P0.05). Sigma 3.5 statistical software analysis). The number of oocysts of Anopheles stephensi treated with Bs was significantly higher than that of the control group (56.69 卤69.29) and (84.19 卤75.64), Bs), respectively (P0.05). Conclusion the sublethal dose of Bs 2362 strain can significantly increase the infection rate and infection degree of Plasmodium yoelii to Anopheles stephensi, and improve the ability of malaria transmission of Anopheles stephensi.
【作者单位】：第三军医大学军事预防医学院热带医学研究所;
【基金】：国家自然科学基金面上项目(81271875) 全军后勤科研“十二五”计划面上项目(CWS12J017)~~
【分类号】：R184.3

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