苦楝叶对钉螺酶组织化学的影响

发布时间：2019-03-30 14:47

【摘要】：目的钉螺经苦楝叶浸提液浸泡后,检测其体内三磷酸腺苷酶、琥珀酸脱氢酶、乳酸脱氢酶和一氧化氮合酶的活性,探讨苦楝叶杀螺机制。方法经苦楝叶浸提液处理48h的钉螺,冰冻切片,使用酶组织化学染色检测4种酶活性的变化,实验设未经药物处理对照组。结果实验组钉螺头足部和消化腺的Ca2+-ATPase灰度值分别为0.197±0.059和0.233±0.037,与对照组0.298±0.041和0.413±0.057比较差异有统计学意义(t=9.036和8.594,P0.05);实验组钉螺头足部肌纤维的SDH灰度值为0.152±0.038,与对照组0.228±0.041比较差异有统计学意义(t=7.167,P0.05);实验组钉螺神经中枢、足部和心脏的NOS灰度值分别为0.616±0.064、0.431±0.043和0.716±0.058,与对照组分别为0.514±0.049、0.302±0.042和0.503±0.045比较差异均有统计学意义(t分别为3.833、6.125和8.608,P0.05)。结论经苦楝叶浸提液处理的钉螺NOS水平升高Ca2+-ATPase和SDH水平下降。因此推测钉螺苦楝叶杀螺机制可能是NOS升高增加了钉螺头足部NO的含量,从而抑制头足部有氧呼吸,降低钙泵的活性,引起钙超载,诱导细胞凋亡,最终导致钉螺死亡。
[Abstract]:Objective to detect the activities of adenosine triphosphate, succinate dehydrogenase, lactate dehydrogenase and nitric oxide synthase in Oncomelania hupensis after soaking in the extracts of Melia azadirachta leaves, and to explore the snail killing mechanism of the leaves of Oncomelania hupensis (Oncomelania hupensis). Methods Oncomelania hupensis was treated with extracts of leaves of Melia azadirachta for 48 hours, frozen sections and enzyme histochemical staining were used to detect the changes of four enzyme activities. The control group was set up without drug treatment. Results the gray values of Ca2-ATPase in the cephalopod and digestive gland of the experimental group were 0.197 卤0.059 and 0.233 卤0.037, respectively, which were significantly higher than those of the control group (0.298 卤0.041 and 0.413 卤0.057, P0.05). The SDH gray value of the muscle fibers in the head and foot of the experimental group was 0.152 卤0.038, which was significantly higher than that of the control group (0.228 卤0.041) (t = 7.167, P0.05). The gray values of NOS in the nerve center, foot and heart of the experimental group were 0.616 卤0.064, 0.431 卤0.043 and 0.716 卤0.058, respectively. Compared with the control group, the difference was 0.514 卤0.049, 0.302 卤0.042 and 0.503 卤0.045, respectively (t = 3.833, 6.125 and 8.608, P0.05). Conclusion the NOS level of Oncomelania hupensis treated with Melia azadirachta leaf extract increased the levels of Ca2-ATPase and SDH decreased. It is suggested that the mechanism of snail killing may be that the increase of NOS increases the content of NO in the head and foot of Oncomelania hupensis, which can inhibit the aerobic respiration of the head and foot, decrease the activity of calcium pump, cause calcium overload, induce cell apoptosis and finally lead to the death of Oncomelania hupensis.
【作者单位】：中国疾病预防控制中心寄生虫病预防控制所世界卫生组织疟疾、血吸虫病和丝虫病合作中心卫生部寄生虫病重点实验室;安徽省林业科学研究院;
【基金】：国家“十二五”国家科技计划项目(No.2011BAD38B070101) 国家科技重大专项(No.2012ZX10004220,2008ZX10004-011)
【分类号】：R184.38

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