不同内镜洗涤剂对内镜管腔大肠杆菌生物膜清除力比较
发布时间：2019-06-21 13:00
【摘要】： 目的:比较三种含酶及非含酶洗涤剂对内窥镜管腔生物膜的清除效力。方法:大肠肝菌菌液持续灌流特富龙管10天形成生物膜结构后以三种不同洗涤剂冲洗管腔,然后以环境扫描电镜和和菌落计数两种方法共同评价残余生物膜。结果:三种不同内镜洗涤剂可在不同程度上对特富龙管壁上堆积的细菌生物膜产生瓦解作用,含酶洗涤剂Detergezyme和非含酶洗涤剂Intercept冲洗后的特富龙材料生物膜平均菌落数分别为(3.25±2.83)×10CFU/片和0,与空白对照组中以灭菌蒸馏水冲洗后的生物膜菌落数(3.40±2.23)×10~3CFU/片相比有显著性差异(p＜0.05),含酶洗涤剂Rapid multi-enzyme cleaner冲洗后的生物膜平均菌落计数与对照组相比无显著性差异,电镜观察结果显示Intercept和Detergezyme冲洗后的生物膜结构稀少,而Rapid multi-enzyme cleaner组残留较多生物膜结构。结论:有酶或无酶不是评判内镜洗涤剂清洗效果好坏的标准,临床工作中内镜管腔的预清洗及选用合适的洗涤剂对于内镜的洗消工作非常重要。
[Abstract]:Objective: to compare the scavenging effect of three kinds of enzyme-containing and non-enzyme detergents on endoscope biofilm. Methods: after the biofilm structure was formed by continuous perfusion of coliform bacteria solution for 10 days, the biofilm was washed with three different detergents, and then the residual biofilm was evaluated by environmental scanning electron microscope (EEM) and colony counting. Results: three kinds of endoscopic detergents could disintegrate the bacterial biofilm accumulated on the wall of terflon tube to varying degrees. The average colony number of biofilm of terflon material washed with enzyme detergent Detergezyme and non-enzyme detergent Intercept was (3.25 卤2.83) 脳 10CFU/ and 0, respectively. Compared with the blank control group, there was significant difference in the number of biofilm colonies after washing with sterilized distilled water (3.40 卤2.23) 脳 10~3CFU/ tablets. There was no significant difference in the average colony count of biofilm after washing with enzyme detergent Rapid multi-enzyme cleaner compared with the control group. The results of electron microscope observation showed that the biofilm structure after washing with Intercept and Detergezyme was rare, while the residual biofilm structure in Rapid multi-enzyme cleaner group was more than that in control group. Conclusion: enzyme or no enzyme is not the standard to evaluate the cleaning effect of endoscopic detergent. In clinical work, the pre-cleaning of endoscopic cavity and the selection of appropriate detergent are very important for endoscopic cleaning.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R187
本文编号：2504097
[Abstract]:Objective: to compare the scavenging effect of three kinds of enzyme-containing and non-enzyme detergents on endoscope biofilm. Methods: after the biofilm structure was formed by continuous perfusion of coliform bacteria solution for 10 days, the biofilm was washed with three different detergents, and then the residual biofilm was evaluated by environmental scanning electron microscope (EEM) and colony counting. Results: three kinds of endoscopic detergents could disintegrate the bacterial biofilm accumulated on the wall of terflon tube to varying degrees. The average colony number of biofilm of terflon material washed with enzyme detergent Detergezyme and non-enzyme detergent Intercept was (3.25 卤2.83) 脳 10CFU/ and 0, respectively. Compared with the blank control group, there was significant difference in the number of biofilm colonies after washing with sterilized distilled water (3.40 卤2.23) 脳 10~3CFU/ tablets. There was no significant difference in the average colony count of biofilm after washing with enzyme detergent Rapid multi-enzyme cleaner compared with the control group. The results of electron microscope observation showed that the biofilm structure after washing with Intercept and Detergezyme was rare, while the residual biofilm structure in Rapid multi-enzyme cleaner group was more than that in control group. Conclusion: enzyme or no enzyme is not the standard to evaluate the cleaning effect of endoscopic detergent. In clinical work, the pre-cleaning of endoscopic cavity and the selection of appropriate detergent are very important for endoscopic cleaning.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R187
【参考文献】
相关期刊论文 前2条
1 ;卫生部关于印发《内镜清洗消毒技术操作规范(2004年版)》的通知[J];中华人民共和国卫生部公报;2004年04期
2 刘晓岚;宋志军;陈一强;王立赞;孔晋亮;王恒壮;闫萍;;群体感应系统缺陷对大鼠铜绿假单胞菌慢性肺部感染的影响[J];中华医院感染学杂志;2008年04期
，本文编号：2504097
本文链接：https://www.wllwen.com/yixuelunwen/liuxingb/2504097.html
