综合挑战测试包与自制测试包在医院压力蒸汽灭菌应用研究

发布时间：2019-08-24 07:55

【摘要】：目的过观察3M公司提供的Attest 41382型综合挑战测试包与自制生物监测包在压力蒸汽灭菌器中的灭菌对比,规范高压蒸汽灭菌过程监控的流程、测试装置,确保灭菌效果,降低成本支出,提高工作效率,降低医院感染。方法将挑战包和自制包同时放入高压灭菌锅最难灭菌的底层、近门处排气口上方灭菌,调节不同的参数,分别为常规灭菌参数和模拟失败临界灭菌参数,对比两种灭菌参数下两种测试包的化学指示卡合格率、生物监测合格率及成本和影响因素。结果在常规灭菌参数下,挑战包和自制包灭菌后的包外指示带均变色合格、爬行卡及生物培养合格率均为100.0%;在模拟失败临界灭菌参数下,包外指示带爬行卡合格率为挑战包83.3%,自制包灭菌后为90.0%;生物培养合格率挑战包为63.3%,自制包为80.0%,成品综合挑战测试包和自制测试包在同种灭菌参数条件下灭菌结果差异无统计学意义。结论根据试验证明综合挑战测试包在医院压力蒸汽灭菌应用中符合规范,自制测试包在医院压力蒸汽灭菌中的准确性的影响因素大于成品综合挑战测试包。
[Abstract]:Objective to observe the sterilization of Attest 41382 comprehensive challenge test kit and self-made biological monitoring package in pressure steam sterilizer provided by 3M company, to standardize the process of monitoring high pressure steam sterilization process, to test the device, to ensure the sterilization effect, to reduce the cost, to improve the work efficiency and to reduce the hospital infection. Methods the challenge bag and the self-made bag were put into the bottom layer of the autoclave at the same time, and sterilized above the exhaust port near the door to adjust the different parameters, which were routine sterilization parameters and simulated failure critical sterilization parameters, respectively. The qualified rate of chemical indicator card, the qualified rate of biological monitoring and the influencing factors of the two test kits under the two sterilization parameters were compared. Results under the conventional sterilization parameters, the external indicator band of the challenge bag and the self-made bag were discoloration qualified, the qualified rate of crawling card and biological culture was 100.0%, under the simulated failure critical sterilization parameter, the qualified rate of the crawling card of the outside bag was 83 3%, and that of the self-made bag after sterilization was 90.0%. The qualified rate of biological culture was 63.3%, and that of self-made package was 80.0%. There was no significant difference in sterilization results between finished product comprehensive challenge test package and self-made test package under the same sterilization parameters. Conclusion according to the test, it is proved that the comprehensive challenge test package accords with the standard in the application of hospital pressure steam sterilization, and the influencing factors of the accuracy of the self-made test package in hospital pressure steam sterilization are greater than those of the finished product comprehensive challenge test package.
【作者单位】：四川省骨科医院消毒供应室;四川省骨科医院手术室;
【基金】：四川省卫生厅基金资助项目(120158)
【分类号】：R187

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