建立大鼠急性心肌梗死模型方法的改良

发布时间：2018-01-15 09:02

本文关键词：建立大鼠急性心肌梗死模型方法的改良　出处：《临床心血管病杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的:改良法建立大鼠心肌梗死的模型,提高造模成功率,减少死亡率。方法:60只清洁级SD大鼠,10%水合氯醛注射液腹腔注射麻醉。颈部小切口切开皮肤,暴露气管后利用导丝逆行牵拉技术进行气管插管。呼吸机辅助呼吸,左侧第4肋间进胸,交叉放置两开睑器充分暴露心脏,结扎冠状动脉左前降支。术毕利用注射器膨肺后关胸。4周后处死存活大鼠,取出大鼠心脏,行病理学检查。结果:术中死亡大鼠2只,术后第1天死亡1只,其余57只大鼠存活。4周后肉眼可见心脏标本梗死区心壁变薄,颜色变浅。镜下可见纤维组织排列紊乱、断裂、增生,炎性细胞浸润。提示57只大鼠建立心肌梗死模型成功,成功率93.3%。结论:利用改良法建立大鼠急性心肌梗死模型,死亡率低,造模成功率高。
[Abstract]:Objective: to establish the model of myocardial infarction in rats with modified method, to increase the success rate and reduce the mortality. Methods: 60 SD rats of clean grade were used. 10% chloral hydrate injection intraperitoneal injection anaesthesia. Neck incision incision skin, exposure tracheal trachea after the use of retrograde pull technology tracheal intubation, ventilator assisted breathing, left fourth intercostal chest. The left anterior descending branch of coronary artery was ligated by cross-placing two eyelid openers to fully expose the heart. The surviving rats were killed at the end of the operation by using syringe to expand the lung and close the chest for 4 weeks. The heart of the rats was removed. Results: two rats died during the operation and one died on the first day after operation. The heart wall of the infarcted area of the other 57 rats was thinned with naked eyes after the survival of 4 weeks. Under the microscope, the fibrous tissue was disordered, broken, proliferated and inflammatory cells infiltrated. The results showed that 57 rats were successful in establishing myocardial infarction model. Conclusion: the rat model of acute myocardial infarction established by the improved method has a low mortality rate and a high success rate.
【作者单位】：济宁市第一人民医院心血管外科;
【分类号】：R542.22;R-332
【正文快照】： 近年来我国冠心病发病率越来越高,成为威胁人类健康的主要疾病之一。因此,对冠心病发病机制及治疗等进行研究意义重大。目前常用的心梗模型实验用动物主要有大鼠、兔、及实验用小型猪。大鼠具有品系纯正、组内差异少、价格低廉、饲养方便、侧支循环少、易于造模成功、手术操作

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