丙泊酚对老年大鼠脑颞叶GABAR1和NMDAR2B表达的影响

发布时间：2018-01-15 23:04

本文关键词：丙泊酚对老年大鼠脑颞叶GABAR1和NMDAR2B表达的影响　出处：《临床麻醉学杂志》2015年12期 　论文类型：期刊论文

【摘要】：目的观察腹腔注射丙泊酚后老年大鼠学习记忆变化以及脑左侧颞叶γ-氨基丁酸受体1(GABAR1)和NMDA受体2B(NMDAR2B)表达的变化。方法雄性SD老年大鼠随机分为三组,每组20只。P1和P7组腹腔注射丙泊酚50mg/kg,维持麻醉3h,C组给予相同剂量的乳剂。分别在给药后第1、7天进行Morris水迷宫实验,测试结束后采集大鼠左侧脑颞叶,采用FISH技术和免疫荧光检测GABAR1和NMDAR2B在基因转录以及蛋白表达的变化。结果与C组比较,P1组潜伏时间明显延长,穿越目标次数明显减少(P0.05)。P1组GABAR1mRNA转录和蛋白表达明显增多(P0.05),NMDAR2BmRNA转录和蛋白表达明显减少(P0.05)。结论大鼠在使用丙泊酚后,其大脑的短时记忆受到影响,这种影响可能与大脑颞叶的功能有关,而机制可能与颞叶区域NMDAR2B表达下调而同时出现GABAR1表达上调有关。
[Abstract]:Objective to observe the changes of learning and memory in aged rats after intraperitoneal injection of propofol, and to observe the changes of 纬 -aminobutyric acid receptor 1GABAR1 and NMDA receptor 2BmDAR2B in left temporal lobe of brain. Methods male SD rats were randomly divided into three groups. Each group (n = 20) and P7 group (n = 20) were intraperitoneally injected with propofol 50 mg / kg. The same dose of emulsion was given to group C for 3 h of maintenance anesthesia. The Morris water labyrinth test was carried out on the 7th day, and the left temporal lobe of the brain was collected after the test. The changes of gene transcription and protein expression of GABAR1 and NMDAR2B were detected by FISH and immunofluorescence. The number of crossing targets significantly decreased the GABAR1mRNA transcription and protein expression in group P0.05. P1 increased significantly (P0.05). NMDAR2BmRNA transcription and protein expression decreased significantly (P 0.05). Conclusion the short-term memory of rat brain is affected by propofol. This effect may be related to the function of temporal lobe, and the mechanism may be related to down-regulation of NMDAR2B expression and up-regulation of GABAR1 expression in temporal lobe.
【作者单位】：河北北方学院;
【基金】：河北北方学院自然科学研究计划项目(120176)
【分类号】：R614
【正文快照】： 1969年,Shallice和Warrington报道了一例左侧外侧裂周区皮质受损的患者短时记忆受损;而晚些时候Markowitsch报道了一例切除了影响左侧下顶叶和后上颞叶皮质肿瘤的患者,也同样表现出低于一般水平的短时记忆能力,但2例患者均保留长时记忆能力[1]。这表明颞叶在学习记忆尤其是短

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