华奥胶囊对糖尿病合并早期大血管病变的炎性因子及ICAM-1、VCAM-1和MCP-1的影响

发布时间：2018-01-16 01:23

本文关键词：华奥胶囊对糖尿病合并早期大血管病变的炎性因子及ICAM-1、VCAM-1和MCP-1的影响　出处：《河南中医学院》2015年硕士论文　论文类型：学位论文

【摘要】：目的:通过观察华奥胶囊对糖尿病合并早期大血管病变大鼠模型的体重、血脂、血糖、炎性因子及粘附分子的影响,探讨其对糖尿病大血管的保护作用。方法:以健康雄性SD大鼠为实验对象,随机分为空白对照组和造模组,其中造模组给予高糖、高脂饲料喂养后给予STZ腹腔注射造模。将造模成功后大鼠随机分为模型组、华奥胶囊低剂量组、华奥胶囊高剂量组和通心络胶囊组。治疗组大鼠分别予华奥胶囊悬液和通心络胶囊悬液灌胃,模型组和空白对照组予同等量生理盐水灌胃,灌胃8周后大鼠麻醉分离腹主动脉和胸主动脉,取腹主动脉血和胸主动脉组织,检测体重、血糖、总胆固醇、甘油三酯;通过竞争性放射免疫分析方法测定炎性因子TNF-a、CRP、IL-6水平;采用ELISA(竞争法)方法检测ICAM-1、VCAM-1、MCP-1的表达,观察各组大鼠胸主动脉病理变化。结果:①正常组大鼠活动自如,状态良好;模型组大鼠出现多饮、多尿、多食、体重下降、乏力、倦怠、尾部溃烂愈合差等变现;治疗组大鼠表现较模型组症状轻。②治疗前大鼠体重对比差异无统计学意义(P0.05);治疗后模型组、华奥低剂量组、华奥高剂量组、通心络组体重均与空白对照组比较差异有统计学意义(P0.05);治疗后华奥高剂量组与华奥胶囊低剂量组、模型组、通心络组比较差异均有统计学意义(P0.05)。③各组大鼠造模成功后1周、2周、4周和治疗后8周血糖变化与空白对照组对比明显升高,差异有统计学意义(p0.05);造模后各时期及治疗后华奥高剂量组、模型组、华奥低剂量、通心络组4组血糖水平相互对比差异均无统计学意义(p0.05)。④治疗后华奥高剂量组、华奥低剂量组及通心络组chol、tg均低于模型组,华奥高剂量组、华奥低剂量组与模型组对比差异有统计学意义(p0.05),华奥高剂量组与华奥低剂量、通心络组血脂水平对比差异均有统计学意义(p0.05)。⑤模型组tnf-a、crp及il-6水平均较空白组高,差异有统计学意义(p0.05);各治疗组与模型组对比差异有统计学意义(p0.05);华奥高剂量组与华奥低剂量组及通心络组比较,crp及il-6炎性因子水平均降低,差异有统计学意义(p0.05);华奥高剂量组与通心络组比较,tn-a炎性因子水平降低,差异有统计学意义(p0.05);华奥高剂量组与华奥低剂量组比较,tn-a炎性因子水平虽降低,但差异无统计学意义(p0.05)。⑥模型组icam-1、vcam-1和mcp-1较空白组表达水平高,差异有统计学意义(p0.05);各治疗组与模型组对比差异有统计学意义(p0.05);华奥高剂量组icam-1、vcam-1和mcp-1的表达水平均低于华奥低剂量组,差异有统计学意义(p0.05);华奥高剂量组mcp-1的表达水平虽低于通心络组,但差异无统计学意义(p0.05)。⑦模型组大鼠胸主动脉光镜下病理表现与空白组相比,可见动脉壁结构紊乱,内膜明显增厚,内皮下见较多聚集的泡沫细胞、沉积的脂质,中膜见间质胶原纤维增生。各治疗组病理表现均较模型组有不同程度减轻,其中华奥胶囊高剂量组和通心络组病理改善较明显。结论:①华奥胶囊可改善调节血脂代谢,降低chol和tg水平;对血糖调节影响不大。②华奥胶囊能够降低糖尿病合并大血管病变模型大鼠血清TNF-a、CRP及IL-6水平和胸主动脉组织ICAM-1、VCAM-1、MCP-1的表达,表明华奥胶囊具有大血管保护作用,其保护作用机制可能与减轻炎症反应有关。
[Abstract]:Objective: through the observation of Ao Capsule on diabetic rat model of early atherosclerosis in weight, blood lipid, blood glucose, effects of inflammatory cytokines and adhesion molecules, to explore its protective effect on diabetic vascular. Methods: healthy male SD rats were randomly divided into control group and model group as blank. The model group given high sugar, high fat diet after intraperitoneal injection of STZ rats. After the successful model rats were randomly divided into model group, Ao capsule low dose group, haoao capsule high dose group and Tongxinluo capsule group. Treatment group rats respectively to Austria and Tongxinluo capsule suspension capsule suspension gavage, model group and blank control group treated with normal saline. After 8 weeks, rats were anesthetized from the abdominal aorta and thoracic aorta, abdominal aorta blood and aortic tissue, body weight, blood glucose, total cholesterol, triglyceride;. A competitive radioimmunoassay method for the determination of inflammatory factors TNF-a, CRP, IL-6; using ELISA (Competition Law) method for detection of ICAM-1, VCAM-1, MCP-1 expression and pathology of rats thoracic aorta were observed. Results: the normal group rats freely, in good condition; the rats in model group appeared to drink, polyuria, polyphagia, weight loss, fatigue, fatigue, poor tail ulcerated healing treatment group rats were realized; compared with the model group. The symptoms of light weight difference was not statistically significant compared to rats before treatment, after treatment (P0.05); model group, low dose group of sports, sports in high dose group, body weight and the blank control group, there was significant difference between Tongxinluo group (P0.05); after the treatment of Ao high dose group and AO capsule low dose group, model group, Tongxinluo group were statistically significant (P0.05). After 1 weeks, the rats were 2 weeks, 8 weeks after 4 weeks and treatment The change of blood glucose compared with the blank control group was significantly increased, the difference was statistically significant (P0.05); haoao high dose group, each time after treatment and model group after modeling, haoao low dose Tongxinluo group, blood glucose levels of 4 groups comparison showed no significant difference (P0.05). The treatment of high dose group sports after a cup of low dose group and Tongxinluo group CHOL, TG were lower than the model group, haoao high dose group was statistically significant Ao low dose group and model group differences (P0.05), Austria high dose group and low dose difference through sports, Tongxinluo group lipid levels contrast were statistically significant (P0.05). The model group TNF-a, CRP and IL-6 levels were higher than those in control group, the difference was statistically significant (P0.05); the difference was statistically significant in each treatment group compared with the model group (P0.05); haoao high dose group and low dose group and Austria Tongxinluo group, CRP and IL-6 levels of inflammatory factors are Decreased, the difference was statistically significant (P0.05); haoao high dose group and Tongxinluo group, tn-a level of inflammatory factors decreased, the difference was statistically significant (P0.05); comparison of sports in high dose group and low dose group tn-a Austria, inflammatory factor level is lower, but the difference was not statistically significant (P0.05). The model group ICAM-1, VCAM-1 and MCP-1 compared with control group, the high expression level, the difference was statistically significant (P0.05); the difference was statistically significant in each treatment group compared with the model group (P0.05); haoao high dose group ICAM-1, the expression level of VCAM-1 and MCP-1 were lower than that of Ao low dose group, the difference was statistically significant (P0.05); the expression level of sports in high dose group MCP-1 was lower than that of Tongxinluo group, but the difference was not statistically significant (P0.05). Compared with the light of thoracic aorta of rats in the model group and the pathological changes of the blank group, visible arterial wall structure disorder, significant intimal thickening, endothelium with The accumulation of foam cells, the deposition of lipid in the membrane, see interstitial collagen fiber hyperplasia. The treatment groups were compared with the model group, pathological manifestations were alleviated, the Ao capsule high dose group and Tongxinluo group were improved significantly. Conclusion: Austria capsule can improve blood lipid metabolism, reduce chol and TG the level of blood glucose regulation; little effect. The Ao capsule can reduce diabetic macrovascular disease serum TNF-a model rats, ICAM-1, CRP and IL-6 levels and aortic tissue VCAM-1, MCP-1 expression showed that the Ao capsule has large vascular protective effects and its protective mechanism may be associated with inflammatory reaction.

【学位授予单位】：河南中医学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R285.5

【相似文献】