针刺不同穴位对大鼠顶核神经元自发放电的影响

发布时间：2018-01-21 09:31

本文关键词： 针刺胃运动小脑顶核神经元电活动　出处：《中国中西医结合杂志》2015年04期 　论文类型：期刊论文

【摘要】：目的通过观察针刺不同部位腧穴对小脑顶核(fastigial nucleus,FN)神经元自发放电的影响,探讨不同部位针刺信号是否传入小脑顶核及相应的效应特征。方法 120只SD雄性大鼠,在20%乌拉坦麻醉状态下定位右侧小脑顶核(坐标:AP 11.6 mm,RL 1.0 mm,H 5.6 mm),采用细胞外玻璃微电极记录的方法,记录该神经元自发放电作为基线。采用随机顺序依次针刺足三里、曲池、胃俞、中脘穴,与每次针刺前基线比较,观察对小脑顶核神经元放电的影响。结果与同穴位针刺前比较,针刺足三里、曲池、胃俞、中脘穴过程中FN神经元放电频率均增高(P0.01,P0.05)。中脘穴对FN放电反应率为56.00%(28/50),足三里、曲池、胃俞穴反应率分别为35.00%(21/60)、34.62%(18/52)、36.36%(20/55),中脘穴反应率明显高于其他穴位,差异有统计学意义(F=2.101,P0.05)。结论针刺足三里、曲池、胃俞、中脘穴均可增高小脑顶核神经元自发放电频率,中脘穴对小脑FN具有优势调节作用。
[Abstract]:Objective to observe the effect of acupuncture at different points on spontaneous discharge of fastigial nucleus FN neurons in cerebellar fastigial nucleus. To investigate whether acupuncture signals at different sites were transmitted to the cerebellar parietal nucleus and the corresponding effects. Methods 120 SD male rats were used. The right cerebellar parietal nucleus (RL 1.0 mm H5.6 mm) was located at the level of 20% Uratan anaesthesia (coordinate: AP 11.6 mm / RL 1. 0 mm / h 5. 6 mm). The spontaneous discharge of the neuron was recorded as the baseline by the method of extracellular glass microelectrode recording. The acupuncture points of Zusanli, Quchi, Weishu and Zhongwan were needled in random sequence, compared with the baseline before each acupuncture. Results compared with those before acupuncture at the same acupoint, the frequency of firing of FN neurons at Zusanli, Quchi, Weishu and Zhongwan were all increased in Zusanli, Quchi, Weishu and Zhongwan (P 0.01). The response rate of Zhongwan acupoint to FN discharge was 56.000.The response rate of Zusanli, Quchi and Weishu was 35.00 and 21 / 60, respectively. The response rate of Zhongwan acupoint was significantly higher than that of other acupoints, and the difference was statistically significant (P < 0.05). Conclusion Acupuncture at Zusanli, Quchi, Weishu and Zhongwan points can increase the frequency of spontaneous discharges of neurons in the cerebellar parietal nucleus, and Zhongwan point has a dominant regulatory effect on FN in the cerebellum.
【作者单位】：南京中医药大学第二临床医学院针药结合实验室;山东药品食品职业学院中药系;
【基金】：国家重点基础研究发展计划(“973”)资助项目(No.2011CB505206) 国家自然科学基金资助项目(No.81202744);国家自然科学基金资助项目(No.8133749) 江苏省高校自然科学研究面上项目(No.11KJB360008) 江苏省特色优势学科资助项目江苏省高校青蓝工程优秀科技创新团队资助项目
【分类号】：R245
【正文快照】： 针灸是治疗疾病的常用方法之一,是一种“内病外治”的方式,通过经络及腧穴的传导作用,以及一定的操作手法,调节机体整体平衡来治疗相关疾病。《灵枢·逆顺》言:“上工刺其未生者也”[1],说明了针灸在中医治疗中的地位。针灸的疗效虽然显著,但相关效应规律及其确切机制尚无定论

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