高原低氧环境下大强度运动后增压辅助方法对大鼠血清自由基及相关指标影响的研究

发布时间：2018-01-21 10:26

本文关键词： 高原训练高压氧舱自由基大鼠　出处：《青海师范大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：通过测定大强度运动后进入不同时间、不同压强下增压氧舱恢复后的大鼠血清自由基接相关指标的含量，探讨高原低氧环境下大强度运动后增压辅助方法对大鼠恢复情况的影响，为进一步研究增压辅助方法对运动员疲劳恢复提供研究基础。方法：将56只Wistar大鼠随机分成7组，（1）高原安静组，安静饲养（2）高原训练组，大负荷训练后不用任何方法帮其疲劳恢复（3）高原训练后进入压力为0.2MPa的增压氧舱恢复一小时的增压组（4）高原训练后进入压力为0.2MPa的增压氧舱恢复两小时的增压组（5）高原训练后进入压力为0.3MPa的增压氧舱恢复一小时的增压组（6）模拟海拔4500m低氧训练组，进入模拟海拔4500m大负荷训练后不用任何方法帮其疲劳恢复（7）进入模拟海拔4500m进行低氧训练后再进入压力为0.2MPa的增压氧舱恢复一小时的增压组，正式训练6天结束后第7天将所有大鼠分批进行跑台至力竭，24小时休息后麻醉处死。测定各组大鼠血清中SOD、CAT、GSH-Px、T-AOC、MDA、ROS的浓度。结果：大鼠在海拔2261m的环境下，进行6天的低氧训练后，进增压氧舱压力增加至0.2MPa一小时，会使大鼠血清中SOD浓度升高，MDA浓度降低，ROS浓度降低，CAT、GSH-Px、T-AOC浓度均无显著性差异。大鼠在海拔2261m的环境下，，进行6天的低氧训练后，进增压氧舱压力增加至0.2MPa两小时，会使大鼠血清中SOD浓度升高，CAT浓度下降、MDA浓度降低，GSH-Px、T-AOC、ROS浓度均无显著性差异。大鼠在海拔2261m的环境下，进行6天的低氧训练后，进增压氧舱压力增加至0.3MPa一小时会使大鼠血清中CAT浓度下降，MDA浓度下降，SOD、GSH-Px、T-AOC、ROS浓度均无显著性差异。大鼠在模拟海拔4500m的环境下，进行6天的低氧训练后，进增压氧舱压力增加至0.2MPa一小时，会使大鼠血清中ROS浓度升高，SOD、CAT、GSH-Px、MDA、 T-AOC浓度均无显著性差异。结论：大鼠经海拔2261m环境下进行6天的低氧训练后，分别进入压力0.2MPa一小时或两小时、0.3MPa一小时后均会使血清中自由基浓度下降，对疲劳恢复有积极的作用。大鼠经海模拟海拔4500m环境下进行6天的低氧训练后，进入压力0.2MPa一小时后会使血清中自由基浓度上升，对疲劳恢复有负面作用。大鼠经海拔2261m环境下进行6天的低氧训练后，进增压氧舱压力增加到0.2MP，一小时或两小时，效果最好，压力加到0.3MP，一小时，效果次之。
[Abstract]:Objective: to determine the content of serum free radical grafting indexes in rats after high intensity exercise at different time and pressure after recovery of hyperbaric oxygen chamber. Objective: to investigate the effect of hyperbaric auxiliary method after high intensity exercise on the recovery of rats under high altitude hypoxia. Methods: 56 Wistar rats were randomly divided into 7 groups (n = 7). (2) High altitude training group. After heavy load training, no method is needed to help it recover from fatigue. After high altitude training, it enters the pressurized oxygen chamber with pressure of 0.2 MPA and recovers for one hour. After altitude training, enter the pressurized oxygen chamber with a pressure of 0.2MPa and resume the pressurized oxygen tank for two hours.) after the altitude training, enter the pressurized oxygen chamber with the pressure of 0.3MPa and recover the pressurized oxygen tank for one hour. Simulated altitude 4500m hypoxia training group. Enter the simulated altitude of 4500m heavy load training without any means to help its fatigue recovery. After hypoxic training at simulated altitude of 4500m, the pressurized oxygen chamber with a pressure of 0.2MPa returned to the pressurized group for one hour. On the 7th day after 6 days of formal training, all rats were anesthetized and killed after 24 hours' rest, and the serum GSH-PxX T-AOC of the rats in each group was determined. Results: after 6 days of hypoxic training, the pressure of oxygen chamber increased to 0.2 MPA for one hour. It can increase the concentration of SOD and decrease the concentration of Ros in serum of rats. There was no significant difference in T-AOC concentration. After 6 days of hypoxic training, the pressure of the pressurized chamber increased to 0.2 MPA for two hours at 2261m altitude. It can increase the concentration of SOD in serum of rats and decrease the concentration of cat. The concentration of malondialdehyde (MDA) decreases the concentration of GSH-PxX T-AOC. There was no significant difference in the concentration of ROS. After 6 days of hypoxic training, rats were exposed to hypoxia at an altitude of 2261m. When the pressure of oxygen chamber increased to 0.3 MPA for one hour, the concentration of CAT in serum of rats decreased and the concentration of MDA decreased. There was no significant difference in the concentration of ROS. After 6 days of hypoxic training, the pressure of the pressurized chamber increased to 0.2 MPA for one hour under the simulated altitude of 4500m. The concentration of ROS in serum of rats was increased. There was no significant difference in T-AOC concentration. Conclusion: after 6 days of hypoxic training at 2261m altitude, the rats entered the pressure of 0.2MPa for one hour or two hours, respectively. The concentration of free radicals in serum decreased after 0.3 MPA for one hour, which had a positive effect on the recovery of fatigue. After 6 days of hypoxia training at simulated altitude of 4500m, rats were trained at sea level for 6 days. The concentration of free radicals in serum increased after entering the pressure of 0.2MPa for one hour, which had a negative effect on fatigue recovery. The rats were trained at 2261m altitude for 6 days after hypoxic training. The pressure of oxygen chamber is increased to 0.2 MPP, one hour or two hours, the effect is the best, the pressure is 0.3 MPP, one hour, the effect is second.
【学位授予单位】：青海师范大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：G804.2

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