p38MAPK信号通路在地佐辛减轻大鼠切口痛中的作用

发布时间：2018-02-24 23:00

本文关键词： pMAPK信号通路切口痛地佐辛　出处：《临床麻醉学杂志》2015年01期 　论文类型：期刊论文

【摘要】：目的探讨p38MAPK信号通路在地佐辛减轻大鼠切口痛中的作用。方法雄性SD大鼠32只,体重250~300g,随机均分为四组:假手术组(S组)、切口痛组(I组)、地佐辛0.1mg/kg预处理组(DL组)和地佐辛0.2mg/kg预处理组(DH组)。S组:仅给予麻醉;I组、DL组、DH组建立切口痛模型,其中DL组和DH组在建立模型前30min通过腹腔注射0.1mg/kg和0.2mg/kg的地佐辛,I组建立模型前30min腹腔注射同体积的生理盐水。按Brennan法制成大鼠切口痛模型,以von Frey细丝法、热辐射法观察大鼠术前30min、术后4、8、24h机械刺激缩足反应阈值(MWT)和辐射热刺激缩足反应潜伏期(WTL)。术后24h取相应节段脊髓,并以Western blot法检测脊髓pp38MAPK蛋白表达。结果与S组比较,I、DL和DH组术后4、8、24h的MWT明显降低和WTL明显缩短(P0.05);与I组比较,DL和DH组MWT明显增高和WTL均明显延长(P0.05);与DL组比较,DH组MWT增高和WTL延长,但组间差异无统计学意义。与S组比较,术后24hI、DL和DH组脊髓p-p38MAPK蛋白表达明显增加(P0.05);与I组比较,DL和DH组脊髓pp38MAPK蛋白表达明显降低(P0.05);与DL组比较,DH组p-p38MAPK蛋白表达降低,但组间差异无统计学意义。结论术前腹腔注射地佐辛的抗伤害作用可能与抑制脊髓p-p38MAPK表达有关。
[Abstract]:Objective to investigate the role of p38 MAPK signaling pathway in relieving incision pain in rats with dizosin. Methods 32 male Sprague-Dawley rats were used. The rats were randomly divided into four groups: sham operation group (group S), incision pain group (group I), dizosin 0.1 mg / kg preconditioning group (group D) and dizosin group (0.2 mg / kg preconditioning group) and dh group (group S). In DL group and DH group, intraperitoneal injection of 0.1 mg / kg and 0.2 mg / kg of dizosinone I was used to establish the model 30 minutes before the model was established by intraperitoneal injection of normal saline of the same volume. The rat model of incision pain was established by Brennan method and von Frey filament method was used. The thermal radiation method was used to observe the threshold value of mechanical stimulation of foot contraction response (MWTs) and the latency of radiation heat stimulation of foot contraction reaction in rats 30 minutes before operation and 8 minutes after operation for 24 hours. The corresponding segments of spinal cord were taken out 24 hours after operation, and the corresponding segments of spinal cord were taken out at 24 hours after operation. The expression of pp38MAPK protein in spinal cord was detected by Western blot method. Results compared with group S and group DH, MWT decreased significantly and WTL shortened significantly at 8h after operation (P 0.05), MWT in group I and DH were significantly increased and WTL was significantly prolonged compared with group DL, and DH was significantly longer than that in group DL. MWT increased and WTL prolonged. Compared with group S, the expression of p-p38 MAPK protein in spinal cord increased significantly in IDL and DH groups at 24 h after operation, the expression of p-p38 MAPK protein in spinal cord in group I and group I decreased significantly compared with group I, and the expression of p-p38 MAPK protein in group D was significantly lower than that in group D (P < 0.05), and the expression of p-p38 MAPK protein in group D was significantly lower than that in group D (P < 0.05). Conclusion the anti-nociceptive effect of intraperitoneal injection of dizosin may be related to the inhibition of p-p38 MAPK expression in the spinal cord.
【作者单位】：杭州市第一人民医院麻醉科;浙江大学医学院附属邵逸夫医院麻醉科;浙江大学医学院附属邵逸夫医院普外二科;
【基金】：浙江省教育厅科研项目(Y201121887)
【分类号】：R614

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