中药仙茅对大鼠腓肠肌单收缩张力及mTOR激活效应影响的研究

发布时间：2018-02-25 00:08

  本文关键词： IGF-1 仙茅 腓肠肌 mTOR　出处：《曲阜师范大学》2014年硕士论文　论文类型：学位论文

【摘要】：1.研究目的 通过对灌服中药仙茅制剂后的SD大鼠不同时段的腓肠肌单收缩张力、肌组织IGF-1含量和雷帕霉素靶蛋白(mTOR)mRNA的表达水平的测定，探讨中药仙茅对增强骨骼肌收缩能力的机制和时间效应。 2.研究方法 以9周龄SD雄性大鼠为实验研究对象，随机分为4组，即空白组，灌胃后90min组，灌胃后120min组，灌胃后240min组，每组8只，分笼饲养。经过一周的常规饲养后，对其进行为期2天的灌胃适应，灌注蒸馏水。在第3天，经过12h的禁食禁水之后，对除空白组之外的三组大鼠灌胃中药仙茅制剂，而空白组灌胃等量的蒸馏水。分别在灌胃后90、120和240min对大鼠进行乙醚麻醉后，游离一侧腓肠肌远端，借助BL-420F生物信号采集处理系统测试并记录大鼠腓肠肌单收缩张力，，并取大鼠另一侧腓肠肌入液氮速冻，之后分别采用elisa法和RT-PCR法检测大鼠腓肠肌IGF-1的含量和mTORmRNA的表达。 3.实验结果 3.1与对照组相比，灌药的3组大鼠腓肠肌单收缩张力较空白组显著升高（P0.05），且240min组较其他几组升高最为显著。 3.2灌胃后IGF-1的含量随时间延长显著上升，120min组显著高于90min组（P0.05），240min组极显著高于120min组（P0.01），且灌服中药的3组均明显高于空白组（P0.05）。 3.3空白组的大鼠腓肠肌mTORmRNA表达量显著低于灌药的3组，且240min组显著高于120min组（P0.05），120min组显著高于90min组（P0.05），由90min组到240min组随着时间的推移，mTORmRNA的表达量也呈现逐渐升高的趋势。 4.研究结论： 4.1中药仙茅可明显提高大鼠腓肠肌单收缩张力。 4.2中药仙茅可明显提高大鼠腓肠肌IGF-1的含量，并能促进mTORmRNA的表达，且在服药后的前4个小时之内表达量逐渐上升。 4.3服药后IGF-1的含量上升，mTORmRNA的表达量也出现上升，提示服用仙茅确实对骨骼肌蛋白合成具有一定的促进作用。
[Abstract]:1. Purpose of the study. The contractile tension of gastrocnemius muscle, the content of IGF-1 in muscle tissue and the expression level of rapamycin target protein mTORN mRNA in gastrocnemius muscle of SD rats were measured after administration of Chinese herbal medicine. Objective: to study the mechanism and time effect of Chinese herbal medicine Festuca arundinacea on enhancing skeletal muscle contraction. 2. Research methodology. Sprague-Dawley (SD) male rats aged 9 weeks were randomly divided into four groups: blank group, 90 min group, 120min group and 240 min group. On the third day, after 12 hours of fasting and water ban, three groups of rats, except blank group, were treated with Chinese herbal medicine Festuca arundinacea preparation. In blank group, rats were anesthetized with ether 90,120 and 240 minutes after administration of distilled water, and the contractile tension of gastrocnemius muscle was measured and recorded by BL-420F biological signal acquisition and processing system. The other side of gastrocnemius muscle was frozen with liquid nitrogen, and then the content of IGF-1 and the expression of mTORmRNA in gastrocnemius muscle were detected by elisa and RT-PCR methods respectively. 3. Experimental results. 3.1 compared with the control group, the contractile tension of gastrocnemius in the three groups was significantly higher than that in the blank group, and that in the 240min group was significantly higher than that in the other groups. The content of IGF-1 in 120min group was significantly higher than that in 90min group (P 0.05A 240min group), which was significantly higher than that in 120min group (P 0.01), and the level of IGF-1 in the three groups was significantly higher than that in the blank group (P 0.05). 3.3 the expression of mTORmRNA in gastrocnemius muscle in blank group was significantly lower than that in the control group, and was significantly higher in 240min group than that in 120min group, which was significantly higher than that in 90min group and 90min group. The expression of mTOR mRNA in the group from 90min to 240min showed a trend of increasing with the passage of time. 4. Conclusions of the study:. 4.1 the single contractile tension of gastrocnemius muscle in rats was significantly increased by Festuca arundinacea. 4.2 the content of IGF-1 in gastrocnemius muscle of rats was obviously increased, and the expression of mTORmRNA was increased gradually in the first 4 hours after administration of Festuca arundinacea. 4.3 the content of IGF-1 increased and the expression of mTO mRNA increased, suggesting that citronella did promote skeletal muscle protein synthesis.
【学位授予单位】：曲阜师范大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R285.5

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