七氟醚麻醉对老年小鼠空间认知能力以及海马组织pCreb和Egr1表达的影响

发布时间：2018-03-02 02:15

本文关键词： 海马环腺苷酸应答元件结合蛋白早期生长反应因子空间探索试验老年小鼠七氟醚　出处：《上海交通大学学报(医学版)》2015年08期 　论文类型：期刊论文

【摘要】：目的探讨七氟醚麻醉对老年小鼠空间认知能力以及海马组织中磷酸化环腺苷酸应答元件结合蛋白(pCreb)和早期生长反应因子1(Egr1)表达的影响。方法将12只18月龄老年小鼠随机分为麻醉组和对照组,每组各6只。麻醉组小鼠吸入3%七氟醚4 h,而对照组小鼠吸入100%氧气4 h。水迷宫空间学习记忆能力测量在麻醉后48 h进行,获得训练结束后24 h进行空间探索试验。空间探索试验结束后15 min分离海马组织,通过Western blotting和实时定量PCR方法检测海马组织Egr1、Creb和pCreb的表达。结果麻醉组小鼠第4、5、6日逃逸潜伏期显著长于对照组(P0.05);在空间探索试验中,对照组平台象限时间、路程百分比显著高于麻醉组(P0.01)。与对照组比较,麻醉组小鼠海马组织Egr1的表达量显著下降(P0.05);而两组Creb的表达量无显著差异(P0.05),但麻醉组磷酸化水平下降(P0.05)。结论七氟醚麻醉下调海马组织Egr1表达和Creb磷酸化水平,影响老年小鼠空间认知能力。
[Abstract]:Objective to investigate the effects of sevoflurane anesthesia on spatial cognitive ability and the expression of phosphorylated cyclic adenylate responsive element binding protein (pCreb) and early growth response factor 1 (Egr1) in the hippocampus of aged mice. Rats were randomly divided into anesthetic group and control group. There were 6 mice in each group. The mice in the anesthesia group inhaled 3% sevoflurane for 4 hours, while the mice in the control group inhaled 100% oxygen for 4 hours. The spatial learning and memory ability of the water maze was measured 48 hours after anesthesia. The space exploration experiment was carried out 24 hours after the completion of the training. Hippocampal tissue was isolated from the hippocampus 15 min after the completion of the space exploration test. Western blotting and real-time quantitative PCR were used to detect the expression of Egr1C Creb and pCreb in hippocampal tissue. Results the escape latency of mice in the anesthetized group was significantly longer than that in the control group on 6th, and the time of platform quadrant in the control group was significantly longer than that in the control group. The percentage of distance was significantly higher than that of the anesthetic group (P 0.01). The expression of Egr1 in hippocampal tissue of anesthetized mice was significantly decreased (P 0.05), while the expression of Creb was not significantly different between the two groups, but the level of phosphorylation was decreased in anesthesia group. Conclusion sevoflurane anesthesia can down-regulate the expression of Egr1 and the level of Creb phosphorylation in hippocampus. The spatial cognitive ability of aged mice was affected.
【作者单位】：复旦大学附属上海市第五人民医院麻醉科;
【分类号】：R614

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