评估0.1mm PTFE生物相容性的新西兰兔实验模型

发布时间：2018-03-02 04:01

本文关键词： 心瓣膜生物相容性聚四氟乙烯新西兰兔　出处：《复旦学报(医学版)》2017年04期 　论文类型：期刊论文

【摘要】：目的建立评估0.1 mm聚四氟乙烯(polytetrafluoroethylene,PTFE)新型肺动脉瓣材料生物相容性实验的新西兰兔实验模型。方法选取42只3 kg左右的新西兰成年兔,应用氯胺酮35 mg/kg和右旋美托咪啶0.25 mg/kg肌注麻醉,部分上段开胸,右心室流出道预置荷包,0.1 mm PTFE瓣膜材料经荷包中央小切口植入右心室,收紧荷包并打结完成操作,术后给予吸氧监护直至完全清醒。结果 42只新西兰成年兔接受了手术,死亡6只,其中2只死于麻醉药物的探索阶段,1只死于术后气胸,其余3只分别于术后5天、7天和8天死亡。经解剖发现植入瓣膜材料周围有血栓形成,调整术后抗凝治疗方案后无死亡发生。其余36只新西兰成年兔体重增长,完成整个实验过程。结论新西兰兔适合作为0.1 mm PTFE瓣膜材料生物相容性研究的动物模型,优点是避免了气管插管和体外循环,气胸发生率低。
[Abstract]:Objective to establish a New Zealand rabbit model to evaluate the biocompatibility of a new type of pulmonary valve material containing 0.1 mm PTFE. Methods 42 New Zealand adult rabbits of about 3 kg were selected. Ketamine 35 mg/kg and dexmetidine 0.25 mg/kg were intramuscularly injected into the right ventricle. The upper part of the chest was opened and the right ventricular outflow tract was preset with a 0.1 mm PTFE valve material. The right ventricle was implanted into the right ventricle through a small incision in the center of the purse. After the operation, 42 New Zealand adult rabbits were operated on, 6 of them died, 2 of them died of pneumothorax during the exploratory stage of anaesthesia, and 1 died of pneumothorax after operation. The other 3 died on 5 days, 7 days and 8 days, respectively. Thrombus around the implanted valve material was found, and no death occurred after adjustment of anticoagulant therapy. The remaining 36 New Zealand adult rabbits gained weight. Conclusion New Zealand rabbit is a suitable animal model for biocompatibility study of 0. 1 mm PTFE valve material, which has the advantage of avoiding tracheal intubation and cardiopulmonary bypass, and the incidence of pneumothorax is low.
【作者单位】：复旦大学附属儿科医院心血管中心;
【分类号】：R318.08

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