麦冬多糖-1对心肌缺血再灌注大鼠内皮祖细胞与缺血修饰白蛋白变化的影响

发布时间：2018-03-06 15:27

本文选题：麦冬多糖-　切入点：心肌缺血　出处：《中国老年学杂志》2015年19期 　论文类型：期刊论文

【摘要】：目的探讨麦冬多糖(MDG)-1对心肌缺血再灌注大鼠内皮祖细胞与缺血修饰白蛋白变化的影响。方法选取Wistar大鼠34只,其中心肌缺血再灌注造模成功19只随机分成两组,阴性对照组9只,予以混合饲料和纯净水饲养;治疗组10只,在阴性对照组的饲养基础上,按每天剂量为20 mg/kg MDG-1进行灌胃;正常对照组10只,为未造模的正常大鼠,予以混合饲料和纯净水饲养,由大鼠自行饮食。饲养1 w后麻醉状态下抽取心室血进行镜下内皮祖细胞计数;抽取耳缘静脉血采用白蛋白钴结合(ACB)试验法测定缺血修饰白蛋白;抽完血后处死大鼠,镜下观察心肌梗死区心肌组织病理学变化。结果 1心肌缺血再灌注模型大鼠血中内皮祖细胞水平明显高于正常对照组,经MDG-1治疗后大鼠血中内皮祖细胞水平较未经MDG-1治疗大鼠明显升高(P0.05);2心肌缺血再灌注损伤模型大鼠血中缺血修饰白蛋白含量明显高于正常对照组(P0.05);而MDG-1治疗后的大鼠血中缺血修饰白蛋白水平阴性对照组明显降低(P0.05);3治疗组和正常对照组镜下心肌细胞明显好于阴性对照组,阴性对照组心肌梗死区心肌细胞排列紊乱,可见大量胶原纤维形成;治疗组心肌梗死区心肌细胞排列有序,几乎与正常对照组心肌走形一致。结论 MDG-1能够明显提高心肌缺血再灌注模型大鼠内皮祖细胞水平,降低缺血修饰白蛋白表达,促进血管内皮损伤的修复,改善心肌,对临床具有指导意义,值得临床推广。
[Abstract]:Objective to investigate the effects of Ophiopogon japonicus polysaccharides (MDG) -1 on endothelial progenitor cells (ECs) and ischemic modified albumin (MSA) in myocardial ischemia-reperfusion rats. Methods Thirty-four Wistar rats were selected and 19 of them were randomly divided into two groups. Nine rats in the negative control group were fed with mixed feed and purified water, 10 rats in the treatment group were fed with 20 mg/kg MDG-1 per day on the basis of feeding in the negative control group, and 10 rats in the normal control group were unmodeled normal rats. The rats were fed with mixed feed and purified water and fed on their own diet. After 1 week of feeding, ventricular blood was extracted under anesthesia to count endothelial progenitor cells under microscope, and albumin cobalt combined with ACB-based assay was used to determine the ischemic modified albumin. The myocardial histopathological changes in myocardial infarction area were observed under microscope. Results 1 the level of endothelial progenitor cells in myocardial ischemia reperfusion model rats was significantly higher than that in normal control group. The level of endothelial progenitor cells in the blood of rats treated with MDG-1 was significantly higher than that of rats without MDG-1 treatment, and the content of serum ischemia-modified albumin in rats with myocardial ischemia-reperfusion injury was significantly higher than that in the normal control group, while that after MDG-1 treatment was higher than that in the control group. Compared with the negative control group, the myocardial cells in the control group and the control group were significantly lower than those in the control group, and the myocardial cells in the treatment group and the normal control group were significantly better than those in the negative control group. In the negative control group, the myocardial cells in myocardial infarction area were disordered, and a large number of collagen fibers were formed, while in the treatment group, the myocardial cells in the myocardial infarction area were arranged orderly. Conclusion MDG-1 can significantly increase the level of endothelial progenitor cells, decrease the expression of ischemia modified albumin, promote the repair of vascular endothelial injury and improve myocardium. It has guiding significance to clinic and is worth popularizing.
【作者单位】：潍坊市第二人民医院保健科;
【分类号】：R285.5

【参考文献】