依托咪酯和丙泊酚对新生大鼠认知功能及海马组织影响的研究

发布时间：2018-03-08 14:35

本文选题：依托咪酯　切入点：丙泊酚　出处：《桂林医学院》2014年硕士论文　论文类型：学位论文

【摘要】：目的：观察并对比依托咪酯和丙泊酚对新生大鼠早期空间认知能力、探索能力、学习记忆能力及海马组织中神经元形态、Caspase-3、γ-氨基丁酸的影响。方法：将日龄17-18天的SPF级幼年SD大鼠经电迷宫筛选后选取57只（体重31-40g）随机平均分为3组，每组19只：对照组（单次腹腔注射生理盐水10ml/kg）、依托咪酯组（单次腹腔注射依托咪酯5mg/kg）和丙泊酚组（单次腹腔注射丙泊酚50mg/kg）。对照组腹腔注射生理盐水后3小时及依托咪酯和丙泊酚组麻醉清醒后3h，每组随机选取10只行行为学测试（旷场实验、洞板实验、电迷宫实验）；每组随机选取3只，断头法处死，取海马组织应用HE染色法观察海马神经元的变化和应用免疫组化染色法检测Caspase-3的表达情况；每组随机选取6只，断头法处死，取海马组织利用全自动氨基酸分析仪检测GABA的含量。结果：（1）麻醉前三组大鼠之间的体重比较差异无统计学意义（P＞0.05）；三组大鼠都是经过电迷宫筛选，学习记忆能力相比差异无统计学意义（P＞0.05）。（2）旷场实验测试结果比较：依托咪酯和丙泊酚组大鼠在中央格停留的时间和2min内穿越的格子数（4.40±2.01,4.60±1.96和37.90±11.88,36.30±15.68）与对照组（3.70±1.06,39.10±11.89）相比（P＞0.05），差异无统计学意义。（3）洞板实验测试结果比较：依托咪酯和丙泊酚组大鼠5min内伸入洞的次数（12.00±3.13,10.00±2.79）与对照组（16.30±4.62）比较都减少（P＜0.05），，差异有统计学意义。（4）电迷宫实验测试结果：①依托咪酯组大鼠正确反应次数和全天总反应时间（12.40±2.07,79.10±11.64）与对照组（13.30±2.00,67.70±12.18）比较均有改变（P＞0.05），但差异无统计学意；②丙泊酚组大鼠正确反应次数（9.80±2.39）与对照组比较（P＜0.01）有显著性差异且与依托咪酯组比较（P＜0.05）差异也有统计学意义；丙泊酚组大鼠全天总反应时间（82.30±10.20）与对照组比较（P＜0.05）差异有统计学意义，与依托咪酯组比较（P＞0.05）差异无统计学意义。（5）HE染色结果比较：三组海马CA1区神经元数目和椎体细胞的形态无明显差异，CA1区神经细胞形态正常，排列基本规则，神经元密度均匀，未见明显椎体细胞变性、坏死。（6）Caspase-3免疫组化染色结果比较：海马CA1区神经元Caspase-3表达情况，胞浆中出现棕黄色颗粒为阳性细胞（显微镜倍数×400，高倍镜视野下每个标本观察10个视野，取总和）。依托咪酯和丙泊酚组海马神经元Caspase-3表达的细胞计数分别为（35.67±1.53，37.33±2.31）均高于C组（32.33±2.52），但差异无统计学意义（P＞0.05）。（7）依托咪酯和丙泊酚组海马组织γ-氨基丁酸含量（47.88±10.86，73.14±11.79）都高于对照组（39.08±10.56），但依托咪酯组与对照组比较（P＞0.05）差异无统计学意义，丙泊酚组与对照组比较（P＜0.01）有极显著性差异；丙泊酚组与依托咪酯组比较（P＜0.01）也有极显著性差异。结论：（1）单次腹腔注射5mg/kg依托咪酯和50mg/kg丙泊酚对新生大鼠早期认知功能均有影响，依托咪酯主要表现在探索能力，丙泊酚现在探索能力和学习记忆能力两方面。（2）依托咪酯和丙泊酚对海马组织神经元形态、Caspase-3的表达均无明显影响。（3）丙泊酚增加γ-氨基丁酸的合成和分泌，依托咪酯对γ-氨基丁酸的合成和分泌无明显影响，可能是造成两种药物对新生大鼠早期的认知功能影响差异的原因。
[Abstract]:Objective: To observe and contrast of etomidate and propofol on early exploration ability of spatial cognition, neonatal rat, neuronal morphology, the ability of learning and memory in the hippocampus of Caspase-3. The effect of gamma aminobutyric acid. Methods: 17-18 day old SPF young SD rats by electric maze after screening a total of 57 (weight 31-40g) were randomly divided into 3 groups, 19 rats in each group: control group (single intraperitoneal injection of saline 10ml/kg), etomidate group (single intraperitoneal injection of etomidate and propofol group (5mg/kg) by single intraperitoneal injection of propofol 50mg/kg). The control group 3 hours after intraperitoneal injection of saline and etomidate and propofol group after anesthesia 3h, 10 rats in each group were randomly selected for behavioral test (open field test, hole board test and electric maze experiment); each group of 3 randomly selected rats were killed by decapitation, hippocampus tissues were observed with HE staining of the hippocampal neurons Changes and immunohistochemical staining was used to detect the expression of Caspase-3 in each group were randomly selected; 6 rats were killed by decapitation, hippocampus content by automatic amino acid analyzer for detection of GABA. Results: (1) the difference between the three groups before anesthesia, the body weight of rats was not statistically significant (P > 0.05); three rats are after maze screening, the ability of learning and memory had no significant difference (P > 0.05). (2) compared with the open field test results: etomidate and propofol group rats stay in the central square of time and 2min through the lattice number (4.40 + 2.01,4.60 + 1.96 and 37.90 + 11.88,36.30 + 15.68) and control group (3.70 + 1.06,39.10 + 11.89) compared (P > 0.05), the difference was not statistically significant. (3) comparison of testing results in experimental hole: number of etomidate and propofol in rats of group 5min and extending into the hole (12 + 3.13,10.00 + 2.79) and the control Group (16.30 + 4.62) were decreased (P < 0.05), the difference was statistically significant. (4) test results of electric maze experiment: the correct response times and etomidate group rats all day long the total reaction time (12.40 + 2.07,79.10 + 11.64) and control group (13.30 + 2.00,67.70 + 12.18) is changed significantly (P > 0.05), but no statistical difference; the number of correct responses in the propofol group rats (9.80 + 2.39) compared with the control group (P < 0.01) and there was significant difference compared with etomidate group (P < 0.05) there were significant differences; Propofol group rats all day long the total reaction time (82.30. 10.20) compared with the control group (P < 0.05) the difference was statistically significant, compared with etomidate group (P > 0.05) showed no significant difference. (5) the results of HE staining: comparison of the three groups the number of hippocampal CA1 neurons and pyramidal cells form no significant difference, the morphology of CA1 neurons in normal, row Among the basic rules, the neuron density uniform, no obvious vertebral cell degeneration and necrosis. (6) Caspase-3 immunohistochemical staining results: the expression of Caspase-3 in hippocampus CA1 neurons, the cytoplasm appeared Brown particles as positive cells (400 x magnification, at higher magnification of each sample in 10 visual fields, take total). The expression of etomidate and propofol group Caspase-3 in hippocampus cell count respectively (35.67 + 1.53,37.33 + 2.31) were higher than that of group C (32.33 + 2.52), but the difference was not statistically significant (P > 0.05). (7) etomidate and propofol group hippocampus GABA content (47.88 + 10.86,73.14 + 11.79) are higher than that of the control group (39.08 + 10.56), but etomidate group compared with the control group (P > 0.05) there was no significant difference compared with the control group, propofol group (P < 0.01) has significant differences; Propofol group and etomidate group The comparison (P < 0.01) also had very significant difference.
(1) a single intraperitoneal injection of etomidate and 50mg/kg 5mg/kg effects of propofol on early cognitive function in neonatal rats were mainly manifested in the ability to explore, etomidate, propofol now exploration ability and the ability of learning and memory in two aspects. (2) of etomidate and propofol on hippocampal neuron morphology in Textile Group, had no effect on the expression of Caspase-3 (3). Increased synthesis of GABA and secretion of propofol, no obvious effect of etomidate on GABA synthesis and secretion, may be the cause of the effect of two kinds of drugs on cognitive function in early neonatal rats difference.

【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R614.2

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