反流性食管炎混合反流病证结合大鼠模型的建立与评价

发布时间：2018-03-08 22:03

本文选题：反流性食管炎　切入点：混合反流　出处：《中华中医药杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的:通过多方面评价探索建立一种反流性食管炎(RE)混合反流的病证结合的大鼠模型。方法:雄性SD大鼠随机分为假手术组、模型组、病证结合模型组,每组12只。模型组采用食管十二指肠侧侧吻合术造模,病证结合模型组采用食管十二指肠侧侧吻合术叠加夹尾刺激法的方法,术后通过行为学观察及测量体质量、进食量、饮水量、抓力等综合评价,并对死亡大鼠随即进行尸体解剖;存活动物分别于术后7、14、21d处死进行食管病理组织观察,判断该模型是否成功,并确定模型建立周期。结果:假手术组中麻醉意外死亡3只。术后非预期死亡12只(33.33%),其中模型组死亡6只(16.67%),病证结合模型组死亡6只(16.67%)。尸解分析死亡原因感染、窒息、出血、吻合口瘘和穿孔等。各组存活大鼠均可见不同程度病变形成,且术后生活质量良好。术后第3周,模型组及病证结合模型组大鼠进食量、饮水量、体质量、抓力等方面较假手术组明显下降(P0.01,P0.05),食管HE染色切片显示中性粒细胞浸润、黏膜基底层增厚,食管炎性反应形成。结论:食管十二指肠侧侧吻合术叠加夹尾刺激法所形成的大鼠RE肝胃不和证动物模型制备方法,模型质量及病变形成率高,术后动物并发症及死亡率相对较小。
[Abstract]:Objective: to establish a rat model of combined disease and syndromes of reflux esophagitis and reflux. Methods: male Sprague-Dawley rats were randomly divided into sham operation group, model group and disease syndrome combined model group. There were 12 rats in each group. The model group was made by esophagoduodenal side anastomosis, the disease combined with syndrome model group was treated by esophagoduodenal side anastomosis plus tail clamping stimulation, and the body mass and food intake were measured by behavioral observation after operation. The dead rats were then dissected, and the surviving animals were killed at 714 ~ 21 days after operation to observe the esophageal histopathology, and to judge whether the model was successful or not. Results: in the sham operation group, 3 patients died of anaesthesia accident, 12 of them died unexpectedly after operation. Among them, 6 died in the model group (16. 67g), 6 died in the model group (16. 67m), 6 died in the model group (16. 67m). The causes of death, infection, asphyxia and hemorrhage were analyzed by autopsy. Anastomotic fistula and perforation were observed in all groups, and the quality of life was good after operation. At the 3rd week after operation, the rats in the model group and the model group were fed, drinking water, and body weight. The grabbing power was significantly lower than that in the sham-operated group. The HE staining section of the esophagus showed neutrophil infiltration and thickening of the basal layer of the mucosa. Conclusion: the animal model of RE hepatogastric disharmony caused by esophagoduodenal side anastomosis combined with tail stimulation is of high quality and pathological changes. Postoperative animal complications and mortality were relatively small.
【作者单位】：北京中医药大学;中国中医科学院西苑医院脾胃所;
【基金】：北京市科委十病十药项目(No.Z141100002214012)~~
【分类号】：R-332;R259

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