虎杖苷对非酒精性脂肪肝保护作用的实验研究

发布时间：2018-03-24 21:40

本文选题：非酒精性脂肪肝　切入点：虎杖苷　出处：《中华中医药学刊》2015年05期

【摘要】：目的:探讨虎杖苷治疗非酒精性脂肪肝的疗效。方法:(1)造模:健康雄性SD大鼠47只,随机分为5组:正常组(10只)、模型组(10只)、虎杖苷大剂量组(9只)、虎杖苷小剂量组(9只)和双环醇组(9只)。正常组予标准饲料,其它4组给予高脂饲料;5组共喂养10周后,从正常组与模型组中各随机挑选1只,取肝脏做病理切片,正常组大鼠肝脏切片作对照,证实模型组大鼠非酒精性脂肪肝的形成。(2)治疗:正常组与模型组予以生理盐水灌胃,5 m L/次;虎杖苷大、小剂量组分别以200 mg·kg-1·d-1,100 mg·kg-1·d-1的虎杖苷混悬液灌胃,双环醇组以100 mg·kg-1·d-1的双环醇混悬液灌胃。以上5组皆观察4周,期间各组大鼠统一用标准饲料饲养。次日大鼠禁食12 h后,腹腔麻醉,1抽取腹主动脉血5 m L,分离血清,检测大鼠血清中肝功(丙氨酸氨基转移酶ALT、天门冬氨酸氨基转移酶AST)、血脂(总胆固醇TC、甘油三酯TG);2取肝组织,在大鼠肝脏的同一部位取4块黄豆大小肝组织:一块投多聚甲醛浸泡,为HE染色观察肝脏病理学改变准备。另两块装Ep管,速投液氮。一份观察油红O染色观察肝细胞内脂质沉积状况。结果:1大鼠体质量变化:造模第10周末,模型组、虎杖苷两个剂量组和双环醇组的大鼠体质量明显高于正常组(P0.05,0.01);治疗1周后,与模型组相比虎杖苷大剂量组和双环醇组体质量开始出现降低(P0.05,0.01),治疗第4周末,正常组、虎杖苷两个剂量组及双环醇组大鼠体质量低于与模型组,差异具有统计学意义(分别对应为P0.05,0.01),且虎杖苷大剂量对大鼠体质量减轻效果与双环醇组相当(P0.05)。2肝功血脂的变化:治疗4周后,与模型组相比,TC、TG在虎杖苷两个剂量组、双环醇组中均得到明显改善(P0.05,0.01)。虎杖苷大剂量组对TG的降低作用优于双环醇组(P0.05)。3肝脏病理的变化:治疗4周后,与模型组相比,肝细胞脂肪变、气球样变、小叶炎症和门脉炎症虎杖苷两个剂量组、双环醇组中均得到明显改善。4肝脏油红染色变化:治疗4周后,与模型组相比,可见肝细胞脂肪含量在虎杖苷两个剂量组、双环醇组中均得到明显改善。结论:虎杖苷对非酒精性脂肪肝具有较好疗效,它可以减轻高脂饮食导致的非酒精性脂肪肝大鼠模型的体质量增加、改善血脂肝功的异常、改善脂肪肝病理学改变和肝细胞脂质沉积。
[Abstract]:Objective: to investigate the efficacy of polydatin in the treatment of non-alcoholic fatty liver. They were randomly divided into 5 groups: normal group (n = 10), model group (n = 10), large dose group (n = 9), polydatin group (n = 9) and bicyclic alcohol group (n = 9). One rat was randomly selected from the normal group and the model group, the liver was taken for pathological section, and the normal group was used as the control. It was confirmed that the formation of non-alcoholic fatty liver in the model group: normal group and model group were given normal saline for 5 mL / time, and the large and low dose groups were perfused with 200 mg kg-1 d-1100 mg kg-1 d -1 suspension of polydatin, respectively. The rats in the bicyclic alcohol group were fed with 100mg kg-1 d-1 bicyclic alcohol suspension for 4 weeks, during which the rats in each group were fed with standard diet. After fasting for 12 hours, the abdominal aorta blood was extracted from the rats under intraperitoneal anaesthesia for 5 mL and the serum was isolated. Liver function (alanine aminotransferase alt, aspartate aminotransferase ASTT), total cholesterol (TC) and triglyceride (TGG) were measured in serum of rats. Four pieces of soybean liver tissue were taken from the same part of the rat liver: one was soaked in paraformaldehyde to prepare for HE staining to observe the pathological changes of the liver, the other two were filled with EP tubes. A fraction of oil red O staining was used to observe the lipid deposition in hepatocytes. Results the body mass of rats at 1: 1 was changed at the end of the 10th week of model making, the model group, The body mass of the rats in the two dose groups of polydatin and bicyclol groups was significantly higher than that in the normal group (P0.050.01), and after one week of treatment, the body mass of the high-dose group and bicyclic alcohol group began to decrease compared with the model group, and the body mass of the rats in the high-dose group and bicyclic alcohol group began to decrease, and at the 4th week of treatment, the body mass of the rats in the normal group began to decrease. The body mass of rats in two dose groups of polydatin and bicyclol groups was lower than that in the model group. The difference was statistically significant (corresponding to P0.05P0.01G, respectively, and the effect of high-dose polydatin on reducing body mass in rats was equivalent to that in bicyclol group P0.050.2. the changes of lipids in liver function: after 4 weeks treatment, compared with the model group, TCTG was in the two dose groups of polydatin. The decrease of TG in the high-dose group of polydatin was better than that in the group of dicyclic alcohol: after 4 weeks of treatment, compared with the model group, the fatty and balloon changes of hepatocytes were observed. The changes of liver oil red staining were significantly improved in the two dose groups of polydatin and polydatin in lobular inflammation and portal inflammation. After 4 weeks of treatment, compared with the model group, the fatty content of hepatocytes was found to be in the two dose groups of polydatin. Conclusion: polydatin has a better effect on non-alcoholic fatty liver, it can reduce the increase of body weight and improve the abnormal lipids liver function in rats induced by high-fat diet. Improve fatty liver pathological changes and liver cell lipid deposition.
【作者单位】：中国人民解放军251医院;首都医科大学附属北京中医医院;中国人民解放军军事医学科学院;
【基金】：北京市中医科技重点规划项目(KJTS2011_01) 北京市卫生系统高层次卫生技术人才培养计划项目(2011-2-13) 解放军251医院二类科研项目(KT2014005,KT2015003)
【分类号】：R285.5

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