二甲双胍对DM大鼠血浆VSFT水平和胰岛素抵抗功能的影响

发布时间：2018-03-31 04:29

  本文选题：内脏脂肪素　切入点：二甲双胍　出处：《兰州大学》2014年硕士论文

【摘要】：[目的]1、探索连续小剂量STZ注射复制糖尿病(DM)模型的可行性；2、研究二甲双胍对DM大鼠血浆内脏脂肪素(VSFT)水平的影响；3、探讨内脏脂肪素对胰岛素抵抗(IR)作用的初步机制。 [方法]Wistar大鼠45只,正常饲养1周后,按随机数表示法分为正常对照组(NC,n=15)和糖尿病模型组(n=30)。糖尿病模型组30只大鼠经过连续5天腹腔注射小剂量(30mg/kg) STZ,3天和14天后检测随机血糖,以随机血糖浓度超过16.7mmol/L者为模型复制成功。正常组15只大鼠根据体重剂量连续5天腹腔注射柠檬酸缓冲液(PH=4.5),随后将这30只大鼠再分为糖尿病模型组(DM,n=15)和二甲双胍干预组(MET, n=15).干预组选用二甲双胍作为干预药物,药量200mg·kg-1·d-1灌胃；糖尿病模型组和正常对照组灌胃采用SPF级实验室里面的大鼠常规饮水。三组再同步饲养5周,每周做好检测和记录血糖及体重的工作。模型复制成功5周后,将各组大鼠禁食12h,水合氯醛麻醉后,颈静脉取血,血液以4000转离心,将离心后的血浆液氮速冻并转移到-80℃保存,用于检测血浆VSFT及相关指标等；留取并固定部分肝脏,用于组织学形态检测。 [结果]1、持续小剂量STZ注射可以复制出糖尿病模型,且更接近T2DM型；该大鼠模型发生糖脂代谢紊乱；存在较高的胰岛素抵抗；能够很好的保持高血糖症状态至少5周时间；肝脏发生明显的病理变化。2、对比STZ复制糖尿病模型的其他方法,本方法成功率高；模型复制后动物死亡率低；雌鼠模型复制成功率较雄鼠高；而且模型复制成功后雌鼠的体重消瘦情况较雄鼠明显；模型复制后体重较轻的动物易死亡。3、二甲双胍可以增高DM鼠体重(P0.05)。4、二甲双胍可以降低DM鼠血浆VSFT浓度(P0.05),消弱胰岛素抵抗(P0.05)、消减糖脂代谢紊乱(P0.05)、减轻DM大鼠肝脏受损情况。5、VSFT与体重正相关关系表现在正常大鼠中。6、VSFT与IR正相关性及其与体重的负相关性在DM大鼠身上表现(P0.05)。 [结论]1、持续小剂量STZ注射可复制出DM大鼠模型；2、二甲双胍可降低DM大鼠的VSFT水平,消减胰岛素抵抗；3、VSFT与胰岛素抵抗及体重相关。
[Abstract]:[objective] 1. To explore the feasibility of continuous low dose STZ injection to induce diabetes mellitus (DM) model, to study the effect of metformin on plasma visceral adipoxin (VSFT) level in diabetic rats and to explore the mechanism of visceral lipopolipin on insulin resistance (IRR). [methods] Forty-five Wistar rats were randomly divided into two groups: control group (n = 45) and diabetic model group (n = 30). After 5 consecutive days of intraperitoneal injection of 30 mg 路kg ~ (-1) STZ for 3 days and 14 days, the blood glucose levels were measured in the diabetic model group (n = 30) and the control group (n = 30), respectively, after 1 week of normal feeding, the rats were randomly divided into two groups: the control group (n = 30) and the diabetic model group (n = 30). 15 rats in the normal group were injected citric acid buffer for 5 days according to their body weight. The 30 rats were then divided into diabetic model group (n = 15) and metformin group (n = 15), after which 30 rats were divided into diabetic model group (n = 15) and metformin group (n = 15), after which 30 rats were divided into diabetic model group (n = 15) and metformin group (n = 15). Metformin was used as the intervention drug in the intervention group. The rats in the diabetic model group and the normal control group were given regular drinking water in the SPF laboratory. The three groups were fed simultaneously for 5 weeks, and the blood glucose and body weight were measured and recorded every week. Blood was taken from jugular vein after fasting for 12 hours, blood was collected from jugular vein, blood was centrifuged at 4000 turns, and the plasma was frozen and transferred to -80 鈩，

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