传统藏药“帕珠丸”对大鼠慢性乙醇性肝损伤的保护作用及其机制的研究

发布时间：2018-04-04 08:55

  本文选题：帕珠丸　切入点：乙醇性肝损伤　出处：《青海大学》2014年硕士论文

【摘要】：目的：研究传统藏药“帕珠丸”对慢性乙醇性肝病（alcoholic liver disease,ALD）大鼠肝功能和组织酶活性的作用，以及对PPAR-α1和AMPK-α1基因表达水平的影响，，探讨帕珠丸对慢性乙醇性肝损伤大鼠的保护作用及其可能的机制。 方法：将78只纯系雄性SD大鼠适应性喂养一周后，随机分成正常对照组、模型对照组、联苯双酯组、帕珠丸低、中、高剂量组6组；模型对照组18只，其余每组各12只大鼠。模型组、联苯双酯组、帕珠丸低、中、高剂量组分别给予56%红星二锅头酒10mL·kg-1灌胃；正常组给予10mL·kg-1生理盐水灌胃，每天上午1次，连续灌胃12周，复制乙醇性肝损伤模型。造模成功后，帕珠丸低、中、高剂量组分别给予10mL·kg-1帕珠丸混悬液灌胃，剂量依次为0.05，0.10，0.20g·kg-1；联苯双酯组给予10mL·kg-1联苯双酯滴丸混悬液灌胃，剂量为0.003g·kg-1；正常对照组、模型对照组分别给予同体积生理盐水灌胃，每天上午灌胃1次，连续灌胃3周。24h禁水禁食，麻醉，解剖，取样。检测血清丙氨酸转氨酶（ALT）、天门冬氨酸转氨酶（AST）、甘油三酯（TG）、超氧化物歧化酶（SOD）、还原性谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH-Px）、丙二醛（ADM）含量。并检测肝组织中PPAR-α1mRNA、AMPK-α1mRNA的相对表达水平。 结果：与正常组比较，模型组大鼠肝脏湿重和肝指数和血清中ALT、AST、TG、MDA均明显升高(P＜0.05)，SOD、GSH、GSH-Px均明显降低(P＜0.05)，模型组大鼠肝组织中PPAR-α1mRNA、AMPK-α1mRNA表达量显著降低(P＜0.01)；与模型组比较，帕珠丸各剂量组与联苯双酯组肝脏湿重和肝指数和血清中ALT、AST、TG均明显降低(P＜0.05)，MDA显著降低(P＜0.01)，SOD、GSH、GSH-Px均明显升高(P＜0.05)；与模型组比较，帕珠丸各剂量组与联苯双酯组PPAR-α1mRNA、AMPK-α1mRNA表达量均显著升高(P＜0.05)。结论：帕珠丸能有效保护乙醇对大鼠肝脏的损害，改善肝功能，降低甘油三酯水平，抑制ROS引起的脂质过氧化反应。提示该药对大鼠乙醇性肝损伤有较好的保护作用。
[Abstract]:Objective: to study the effects of traditional Tibetan medicine Pazhuwan on liver function and tissue enzyme activity in rats with chronic alcoholic liver disease (liver) and the expression level of PPAR- 伪 1 and AMPK- 伪 1 genes.To investigate the protective effect of Pazhuwan on chronic ethanol induced liver injury in rats and its possible mechanism.Methods: 78 male Sprague-Dawley rats were randomly divided into normal control group, model control group, biphenyl diester group, pazhu pill low, middle and high dose group, and model control group with 18 rats, 12 rats in each group.Model group, Biphenyl diester group, Pazhuwan low, medium and high dose group were given 56% Hongxing Erguotou 10mL kg-1, and normal group were given 10mL kg-1 saline once a day for 12 weeks, to make ethanol induced liver injury model.After the model was successfully made, Pazu pills were given 10mL kg-1 pellet suspension by stomach in low, medium and high dosage groups, the dosage was 0.05 ~ 0.100.100.20g / kg -1 respectively, the biphenyl diester group was given 10mL kg-1 biphenyl diester dropping pill suspension, the dosage was 0.003g / kg ~ (-1), the normal control group, the control group, the control group, the control group, the control group, the control group, the control group, the control group and the control group, respectively.The model control group was fed with the same volume of normal saline once a day for 3 weeks, 24 hours, anaesthetized, dissected and sampled.Serum alanine aminotransferase (alt), aspartate aminotransferase (AST), triglyceride (TG), superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GSH-PxN), malondialdehyde (MDA) were measured.The relative expression of PPAR- 伪 1 mRNA-AMPK- 伪 1mRNA in liver tissue was detected.缁撴灉:涓庢�ｅ父缁勬瘮杈�,妯″瀷缁勫ぇ榧犺倽鑴忔箍閲嶅拰鑲濇寚鏁板拰琛

本文编号：1709254