黄连阿胶汤加减对糖尿病大鼠降糖疗效及机理的研究

发布时间：2018-04-04 11:28

本文选题：黄连阿胶汤加减　切入点：糖尿病大鼠　出处：《广州中医药大学》2014年硕士论文

【摘要】：目的：研究伤寒论经方治疗糖尿病的疗效及机理。运用中医药基础理论,结合现代医学实验技术,从动物实验研究方面,观察黄连阿胶汤加减对糖尿病大鼠血糖、甘油三脂、胆固醇、胰岛素、胰岛素抵抗指数、瘦素等的影响,分析这些指标在糖尿病中的变化及意义,从而评价黄连阿胶汤加减的降糖疗效及其作用机理,进而促进中药复方制剂治疗糖尿病研究的广度和深度,也将为中医药治疗糖尿病临床疗效的评价和推广提供一个有效的平台。方法：探讨中西医学对糖尿病病因及发病机制的认识,简述中西医药物对糖尿病的治疗机理,对近年来黄连阿胶汤临床应用及实验研究进展进行概况性回顾,总结历代医家对黄连阿胶汤的认识,从而提出“苦酸制甜”法是建立本研究方剂的基础。采用SPF级SD大鼠50只,雌雄各半,随机分为中药组、格列齐特组、模型组、空白组,前3组每组14只,空白组8只。造模组使用STZ按55mg/kg腹腔内注射,造模成功后,中药组按30g/kg、格列齐特组按20mg/kg计算灌胃,空白组、模型组分别灌服等容积蒸馏水,均每日一次,一共灌胃2周。实验期间,各组均予普通饲料。灌胃2周后麻醉大鼠,取血液样本,最后用脱臼法处死大鼠。实验一,研究黄连阿胶汤加减对糖尿病大鼠血糖的影响：评价造模成功与否的随机血糖采用剪尾取血,用快速微量血糖仪进行检测；治疗后空腹血糖的检测采用腹主动脉取血,用全自动生化分析仪检测。实验二,研究黄连阿胶汤加减对糖尿病大鼠血脂、胰岛素、胰岛素抵抗指数、瘦素水平的影响：腹主动脉采血后,甘油三脂、胆固醇用全自动生化分析仪检测,胰岛素、瘦素采用RayBio试剂盒检测,胰岛素抵抗指数公式：HOMA-IR=FPG (mmol/L)×FINS(mU/L)/22.5,计算后取自然对数。结果： 1.大鼠造模组治疗后空腹血糖与空白组相比普遍升高,其中中药组、模型组分别与空白组相比差异具有显著性意义(p0.05),而格列齐特组与空白组相比差异无统计学意义(p0.05)。格列齐特组与模型组相比差异具有显著性意义(p0.05)。中药组虽与模型组相比差异无统计学意义(p0.05),但两组均值差约等于-1。 2.各组大鼠甘油三脂、胆固醇、胰岛素、胰岛素抵抗指数、瘦素组间差异无统计学意义。结论：格列齐特降糖疗效显著。中药组虽与模型组相比差异无统计学意义,但两组均值差约等于-1,同时观察中药组大鼠糖尿病各症状较模型组大鼠为轻,总体情况较前好转,推测中药组可能存在一定的降糖疗效。中药组降糖疗效不理想可能与给药时间过短、样本量少、未能方证对应、药剂制取与灌服药物方法不完善等相关。各组血脂、胰岛素、胰岛素抵抗指数、瘦素指标比较未见明显差异,推测与造模未能诱导明显的胰岛素抵抗相关,因此无法深入探讨该方降糖机制是否与胰岛素抵抗相关。
[Abstract]:Objective:To study the curative effect and mechanism of treating diabetes mellitus with Shufangxuan Meridian prescription.The effects of Huanglian Ejiao decoction on blood glucose, triglyceride, cholesterol, insulin, insulin, insulin resistance index, leptin and so on in diabetic rats were observed by using the basic theory of traditional Chinese medicine and modern medical experimental technology.The changes and significance of these indexes in diabetes mellitus were analyzed to evaluate the hypoglycemic effect of Huanglian Ejiao decoction and its mechanism, and to promote the breadth and depth of the research on the treatment of diabetes mellitus with traditional Chinese medicine compound preparation.It will also provide an effective platform for evaluating and popularizing the clinical efficacy of traditional Chinese medicine in the treatment of diabetes.Methods:To explore the etiology and pathogenesis of diabetes in Chinese and western medicine, to summarize the therapeutic mechanism of traditional Chinese and western medicine on diabetes, and to review the clinical application and experimental research progress of Huanglian Ejiao decoction in recent years.The understanding of Huanglian Ejiao decoction was summarized, and the method of "bitter acid making sweet" was put forward.Fifty SD rats of SPF grade were randomly divided into Chinese medicine group, gliclazide group, model group, blank group, 14 rats in each group, and 8 rats in blank group.The model group was injected intraperitoneally with STZ and 55mg/kg. After the model was successfully made, the Chinese medicine group was given 30 g / kg, the gliclazide group was treated with 20mg/kg, and the blank group and the model group were given equal volume distilled water once a day for 2 weeks.During the experiment, all groups were given common feed.The anesthetized rats were anesthetized for 2 weeks, blood samples were taken, and the rats were killed by dislocated method.Experiment 1, study the effect of Huanglian Ejiao decoction on blood sugar in diabetic rats: the random blood sugar used to evaluate the success of the model was to take blood from the tail and detect it with a rapid micro-blood glucose meter, and the fasting blood glucose was measured by abdominal aorta after treatment.Automatic biochemical analyzer was used to detect.The second experiment was to study the effects of Huanglian Ejiao decoction on blood lipids, insulin, insulin resistance index and leptin level in diabetic rats: after blood collection from abdominal aorta, triglyceride and cholesterol were detected by automatic biochemical analyzer.Leptin was detected by RayBio kit. The insulin resistance index formula was: HOMA-IRFPG / mmol / L) 脳 FINSU / L / L = 22.5. The natural logarithm was calculated.Results:1.After treatment, fasting blood glucose in the model group was generally higher than that in the blank group. There was significant difference between the traditional Chinese medicine group and the model group compared with the blank group, but there was no significant difference between the gliclazide group and the blank group.There was significant difference between Gliclazide group and model group (P 0.05).Although there was no significant difference between the traditional Chinese medicine group and the model group, the difference between the two groups was about-1.2.There was no significant difference in triglyceride, cholesterol, insulin, insulin resistance index and leptin.Conclusion:Gliclazide has a remarkable effect on reducing glucose.Although there was no significant difference between the Chinese medicine group and the model group, the difference between the two groups was about -1.At the same time, the symptoms of diabetes in the traditional Chinese medicine group were lighter than those in the model group, and the overall situation was improved.It is speculated that the Chinese medicine group may have some hypoglycemic effect.The hypoglycemic effect of traditional Chinese medicine group may be related to the short time of administration, the small sample size, the lack of prescription syndrome, the preparation of medicine and the imperfection of the method of drug administration.There was no significant difference in serum lipids, insulin, insulin resistance index and leptin index in each group. It was speculated that there was no obvious relationship between insulin resistance and insulin resistance induced by model making, so it was impossible to explore whether the hypoglycemic mechanism of the prescription was related to insulin resistance.
【学位授予单位】：广州中医药大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R285.5

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