AM251对糖尿病视网膜病变大鼠ICAM-1及VEGF表达的影响

发布时间：2018-04-10 02:25

本文选题：糖尿病　切入点：视网膜病变　出处：《中国老年学杂志》2015年14期

【摘要】：目的探讨大麻素1受体拮抗剂AM251对糖尿病视网膜病变大鼠视网膜细胞间黏附因子(ICAM)-1和视网膜血管内皮生长因子(VEGF)蛋白表达的影响。方法将SD大鼠随机分为正常对照组、糖尿病模型组,正常对照组又分为正常生理盐水对照组(NC组)、正常AM251干预组(NC-C组),糖尿病模型组又分为糖尿病视网膜病变组(DR组)、AM251干预组(DR-C组)。糖尿病模型组予腹腔注射链脲佐菌素55 mg/kg复制糖尿病视网膜病变模型.AM251干预组给予AM 251 0.5 mg/kg腹腔注射,1次/d,连续12 w,其余两组给予等量生理盐水腹腔注射。每周监测血糖、体质量,AM251干预12 w后通过直接及间接眼底镜观察眼底;水合氯醛麻醉下取眼,心脏取血,免疫组织化学法检测视网膜VEGF的变化、Western印迹法分别检测ICAM-1、VEGF的变化,ELISA法测视网膜组织ICAM-1、VEGF的变化及血清中s ICAM-1的变化。结果与正常对照组相比,模型组VEGF、ICAM-1水平明显升高(P0.05),经AM251干预后,DR-C组VEGF、ICAM-1较DR组降低(P0.05),较正常组仍升高(P0.05)。结论AM251可减少糖尿病视网膜病变大鼠视网膜ICAM-1、VEGF的表达,改善视网膜的超微结构,这可能是对糖尿病视网膜病变的保护机制之一。
[Abstract]:Objective to investigate the effects of cannabinin-1 receptor antagonist (AM251) on the expression of ICAM-1 and VEGF protein in diabetic retinopathy rats.Methods SD rats were randomly divided into normal control group and diabetic model group.The normal control group was divided into normal saline control group (NC group), normal AM251 intervention group (NC-C group) and diabetic model group (DM model group).Diabetic retinopathy model was induced by intraperitoneal injection of streptozotocin 55 mg/kg. AM251 intervention group was given AM251 0.5 mg/kg intraperitoneal injection for 12 weeks, and the other two groups were given the same amount of normal saline intraperitoneal injection.Blood glucose was monitored weekly. After 12 weeks of intervention, the fundus was observed by direct and indirect fundus endoscopy, and the blood was taken from the heart under chloral hydrate anesthesia.The changes of VEGF in retina were detected by immunohistochemistry. The changes of VEGF in retina were detected by Western blotting. The changes of VEGF in retina and the changes of s ICAM-1 in serum were detected by Elisa.Results compared with the normal control group, the level of VEGFU ICAM-1 in the model group was significantly higher than that in the normal control group. After the intervention of AM251, the level of VEGFU ICAM-1 in the DR-C group was lower than that in the Dr group, and it was still higher than that in the normal group.Conclusion AM251 can reduce the expression of ICAM-1 and improve the ultrastructure of retina in diabetic retinopathy rats, which may be one of the protective mechanisms for diabetic retinopathy.
【作者单位】：徐州医学院研究生学院;徐州医学院附属医院内分泌科;
【基金】：江苏省科技创新与成果转化(自然科学基金)专项引导资金项目(No.BK2010181)
【分类号】：R587.2

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