帕瑞昔布对肝部分切除术老年大鼠海马炎症反应的影响

发布时间：2018-04-26 07:01

本文选题：帕瑞昔布 + COX-　；参考：《临床麻醉学杂志》2017年03期

【摘要】：目的研究帕瑞昔布对肝部分切除术老年大鼠海马炎症反应的影响。方法选择SPF级雄性SD大鼠70只,20月龄,体重500~600g,随机分为三组:对照组(C组,n=10),手术组(S组,n=30)及帕瑞昔布组(P组,n=30)。采用肝部分切除术建立手术创伤导致海马炎症反应模型。P组大鼠在异氟醚麻醉下行肝部分切除术,并于术前1h腹腔注射帕瑞昔布10 mg/kg,术后每日腹腔注射帕瑞昔布2mg/kg直至处死。S组大鼠在异氟醚麻醉下行肝部分切除术,术前1h腹腔注射与P组等量的生理盐水。C组腹腔注射与以上各组等量的生理盐水。干预后第1、3、7天处死大鼠,取海马组织,采用实时定量PCR法检测环氧合酶-2(COX-2)mRNA的表达量,Western blot法检测COX-2、半胱氨酸蛋白酶-3(caspase-3)的蛋白表达量,ELISA法检测前列腺素E2(PGE2)的含量。结果干预后第1、3天S组和P组COX-2mRNA及蛋白表达、PGE2含量、caspase-3蛋白表达明显高于C组(P0.05或P0.01),但P组明显低于S组(P0.05或P0.01)。干预后第7天三组大鼠COX-2mRNA及蛋白表达、PGE2含量、caspase-3蛋白表达差异无统计学意义。结论肝部分切除术可导致海马炎症反应,帕瑞昔布可显著抑制此种炎症反应,其机制可能与下调海马COX-2mRNA和蛋白表达、PGE2含量和caspase-3蛋白表达有关。
[Abstract]:Objective to study the effect of paroxib on hippocampal inflammation in elderly rats after partial hepatectomy. Methods 70 male Sprague-Dawley rats of SPF grade were randomly divided into three groups: control group C (n = 10), operation group S (n = 30) and paroxib group P (n = 30). Partial hepatectomy was used to establish the model of hippocampal inflammation induced by surgical trauma. Group P was subjected to partial hepatectomy under isoflurane anesthesia. The rats in group S were given partial hepatectomy under isoflurane anesthesia after intraperitoneal injection of paroxib (10 mg / kg) 1 h before operation and daily intraperitoneal injection of paroxib (2mg/kg) until the rats in group S were killed, and the rats in group S received partial hepatectomy under isoflurane anesthesia. Intraperitoneal injection of the same amount of saline as that of group P was performed 1 hour before operation. Group C was injected intraperitoneally with the same amount of saline as those in the above groups. The rats were killed on the 7th day after intervention. The hippocampal tissues were taken out and the expression of cyclooxygenase-2 and caspase-3 were detected by real-time quantitative PCR method. Western blot was used to detect the protein expression of COX-2 and cysteine protease -3caspase-3. The content of prostaglandin E2PGE2 was detected by Elisa. Results on the 1st day after intervention, the expression of COX-2mRNA and protein in group S and group P were significantly higher than those in group C (P0.05 or P0.01), but in group P was significantly lower than that in group S (P0.05 or P0.01). On the 7th day after intervention, there was no significant difference in the expression of COX-2mRNA and PGE2 and caspase-3 protein in the three groups. Conclusion partial hepatectomy can induce hippocampal inflammation, which can be significantly inhibited by paroxib, which may be related to the down-regulation of the expression of COX-2mRNA and protein in hippocampus and the expression of caspase-3 protein.
【作者单位】：武汉大学中南医院麻醉科;
【基金】：国家自然科学基金青年基金(30901393)
【分类号】：R614

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