脑室内注射瘦素对大鼠迷走复合体瘦素受体表达的影响

发布时间：2018-04-29 11:47

  本文选题：瘦素 + 瘦素受体　； 参考：《山东医药》2017年17期

【摘要】：目的探讨脑室内注射瘦素后大鼠迷走复合体瘦素受体(Ob-R)表达的变化。方法将SD大鼠24只随机分为对照组和瘦素干预1 h组(A组)、3 h组(B组)、5 h组(C组),每组6只。给药前1周行侧脑室置管,置管1周后无麻醉状态下对照组侧脑室注射生理盐水,A、B、C组侧脑室注射瘦素,剂量均为3.5μg/μL。实验结束后取脑组织,用HE染色进行迷走复合体定位,用免疫组化方法测定Ob-R在迷走复合体的表达。结果 A、B、C组Ob-R阳性细胞数分别为(12.49±1.66)、(12.54±2.16)、(12.89±1.86)个,对照组阳性细胞数为(5.66±1.04)个;A、B、C组Ob-R阳性细胞光密度积分分别为(28.41±3.60)、(26.18±6.17)、(24.56±4.14)分,对照组为(16.02±2.06)分;与对照组比较,A、B、C组Ob-R阳性细胞数、阳性细胞光密度积分增多(P均0.05)。A、B、C三组间Ob-R阳性细胞数、阳性细胞光密度积分比较差异无统计学意义(P均0.05)。结论瘦素中枢干预后可刺激迷走复合体上Ob-R的表达上调,瘦素刺激时间与Ob-R水平无关。
[Abstract]:Objective to investigate the changes of leptin receptor (Ob-R-) expression in rat vagal complex after intracerebroventricular injection of leptin. Methods Twenty-four Sprague-Dawley rats were randomly divided into two groups: control group (n = 6) and leptin intervention group (n = 6). One week before administration, ventricular catheters were placed in the lateral ventricle. In the control group, the rats in the control group were injected with leptin at the dose of 3.5 渭 g / 渭 L under the condition of no anaesthesia. At the end of the experiment, the brain tissue was taken, and the localization of vagus complex was carried out by HE staining. The expression of Ob-R in the vagal complex was detected by immunohistochemical method. Results the number of Ob-R positive cells was 12.49 卤1.66 卤1.86 in group A and 5.66 卤1.04 in control group, respectively, and the optical density score of Ob-R positive cells in group C was 28.41 卤3.6026.18 卤6.175.56 卤4.14, and the number of Ob-R positive cells in group A and C was 16.02 卤2.06, respectively. The number of Ob-R positive cells and the difference of optical density scores of positive cells between three groups were not statistically significant (P < 0.05). Conclusion leptin central intervention can up-regulate the expression of Ob-R on the vagal complex, and leptin stimulation time is not related to the level of Ob-R.
【作者单位】： 承德医学院附属医院;承德医学院解剖教研室;
【基金】：河北省科学技术研究与发展计划项目(08276101D-20)
【分类号】：R965
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本文编号：1819786