气管内注入左氧氟沙星注射液对大鼠气管粘膜和肺组织的损伤及氨溴索的保护作用

发布时间：2018-04-30 05:13

本文选题：左氧氟沙星注射液 + 纤毛　；参考：《福建医科大学》2014年硕士论文

【摘要】：目的研究气管内注入左氧氟沙星注射液后大鼠气管粘膜纤毛受损情况和肺组织病理改变及肺组织中IL-6蛋白的表达变化，明确气管内注入左氧氟沙星注射液对大鼠气管粘膜超微结构和肺组织的损伤及盐酸氨溴索注射液对这种损伤的保护作用。方法将健康雄性清洁级SD大鼠90只随机分为三组，分别为正常对照组30只，，左氧氟沙星组30只，左氧氟沙星+氨溴索干预组30只。大鼠腹腔麻醉后，左氧氟沙星组、左氧氟沙星+氨溴索干预组各大鼠按2.8mg/100g经环甲膜穿刺向气管内一次性缓慢注入左氧氟沙星注射液，氨溴索干预组在向气管内注入左氧氟沙星注射液前6h按70mg／kg向腹腔内一次性注入盐酸氨溴索注射液。气管内注药后2小时、1天、2天、3天、5天、7天6个不同时间点分别放血处死5只大鼠，迅速剖取大鼠中下段气管及右肺后叶组织。电镜观察中下段气管纤毛的受损情况，光镜观察右肺后叶组织病理变化，免疫组化方法检测肺组织IL-6蛋白的表达情况。结果 1.气管粘膜纤毛的损伤 1.1观察正常对照组纤毛排列整齐、致密，无倒伏、水肿、坏死脱落，左氧氟沙星组和氨溴索干预组除第7天外，其余各时间点纤毛均出现不同程度倒伏、水肿、坏死脱落。 1.2左氧氟沙星组在第2小时、1天、2天、3天、5天与正常对照组比较，气管粘膜纤毛均存在不同程度损伤，具有显著性差异（P0.05）。 1.3左氧氟沙星组中，随着时间的推移，气管粘膜纤毛损伤程度逐渐加重，在气管内给药后第3天时受损程度达最严重程度，各时间点间均具有统计学差异（P0.05）。 1.4氨溴索干预组在第2小时、2天、3天、5天时纤毛损伤程度与左氧氟沙星组比较，均有所减轻（P0.05）。 2.肺组织病理改变 2.1正常对照组支气管上皮细胞排列整齐，支气管腔内无渗出物。左氧氟沙星组出现不同程度支气管上皮细胞破坏，肺泡间隔内可见明显炎性细胞浸润，肺泡腔内出现渗出液、红细胞、炎症细胞，第7天基本恢复正常。 2.2左氧氟沙星组各时间点肺组织损伤程度比较中，第1天明显重于第2小时（P0.05），第2天达到最严重程度，第3天起开始下降，第7天基本恢复正常。 2.3氨溴索干预组各时间点中，在第2小时、2天、3天、5天与左氧氟沙星比较，肺组织损伤均有明显减轻（P0.05）。 3.IL-6蛋白的表达变化 3.1正常对照组肺组织IL-6蛋白少量表达，主要表达于支气管上皮细胞细胞质中，染色呈淡黄。左氧氟沙星组除第7天外，其余各时间点与正常对照组比较均具有显著性差异（P0.05）。 3.2左氧氟沙星组从第1天起大部分支气管上皮细胞细胞质均染色，呈棕褐色，第5天起染色变浅，第7天着色呈淡黄色。 3.3氨溴索干预组第1天、7天IL-6蛋白表达量与左氧氟沙星组相比无统计学意义，其余各时间点比较均有明显减少（P0.05）。结论 1.气管内注入左氧氟沙星注射液对大鼠气管粘膜和肺组织均能产生损伤作用，引起肺组织IL-6蛋白表达增加，IL-6蛋白表达增加可能参与了这种损伤过程。 2.盐酸氨溴索注射液对保护气管粘膜和肺组织的损伤有一定的作用，能降低肺组织IL-6蛋白的表达量。
[Abstract]:objective
The damage of trachea mucociliary, pathological changes of lung tissue and the expression of IL-6 protein in lung tissue were studied in rats after intratracheal injection of Levofloxacin Injection, and the damage of tracheal ultrastructure and lung tissue in the trachea and the protection of Ambroxol Hydrochloride Injection on the injury by intratracheal injection of Levofloxacin Injection were studied. Action. Method
90 healthy male clean SD rats were randomly divided into three groups, 30 in the normal control group, 30 in the levofloxacin group and 30 in the left ofloxacin + ambroxol intervention group. After the abdominal anesthesia, the left ofloxacin group and the left ofloxacin + ambroxol intervention group were injected into the trachea by 2.8mg/ 100g through the paracentesis to the trachea at one time. Levofloxacin Injection, ambroxol intervention group injected Levofloxacin Injection into the abdominal cavity before injection of Levofloxacin Injection into the trachea and injected Ambroxol Hydrochloride Injection into the abdominal cavity at one time. After 2 hours, 1 days, 2 days, 3 days, 5 days, 7 days and 6 different time points, 5 rats were injected into the trachea, and the middle and lower middle trachea and right lung of the rat were quickly dissected. The damage of the endotracheal cilium in the middle and lower middle segment was observed by electron microscopy. The pathological changes of the posterior lobe of the right lung were observed by light microscopy. The expression of IL-6 protein in the lung tissue was detected by immunohistochemical method.
Result
1. injury of trachea mucociliary
1.1 the cilia in the normal control group was arranged neatly, dense, no lodging, edema, necrosis and shedding, and the left ofloxacin group and ambroxol intervention group had different degrees of lodging, edema, necrosis and shedding in the other time points except seventh days.
1.2 levofloxacin group in second hours, 1 days, 2 days, 3 days, 5 days compared with the normal control group, the tracheal cilium had different degrees of damage, with significant difference (P0.05).
In 1.3 levofloxacin group, the damage degree of cilium in trachea mucous membrane was gradually increased with time, and the degree of damage reached the most serious degree at third days after administration in trachea, and there was statistical difference between each time point (P0.05).
1.4 in the ambroxol intervention group, the ciliary damage was reduced in second hours, 2 days, 3 days, 5 days compared with levofloxacin group (P0.05).
2. pathological changes of lung tissue
2.1 the bronchial epithelial cells in the normal control group were arranged neatly and there were no exudates in the bronchoalveolar cavity. There were different degrees of bronchial epithelial cell destruction in the levofloxacin group, obvious infiltration of inflammatory cells in the alveolar septum, exudation in the alveolar cavity, red blood cells and inflammatory cells, and the seventh days were basically restored to normal.
The lung tissue damage at each time point of 2.2 levofloxacin group was compared in the first days (P0.05), the second day was the most serious, the third days began to decline, and the seventh days were basically restored to normal.
2.3 in Ambroxol intervention group, lung injury was significantly reduced at second hours, 2 days, 3 days, 5 days compared with levofloxacin (P0.05).
Expression changes of 3.IL-6 protein
3.1 the expression of IL-6 protein in the lung tissue of the normal control group was mainly expressed in the cytoplasm of the bronchial epithelial cells, and the staining was yellowish. The left ofloxacin group was significantly different from that of the normal control group (P0.05) except for the normal control group (P0.05).
3.2 from the first day, the cytoplasm of most of the bronchial epithelial cells was stained brown, fifth days, and the coloring was pale yellow in seventh days.
3.3 on the first day of ambroxol intervention group, the expression of IL-6 protein was not significantly different from that of levofloxacin group on the 7 day, while the rest time points decreased significantly (P0.05).
conclusion
1. intratracheal injection of Levofloxacin Injection can damage the trachea mucous membrane and lung tissue of rats. The expression of IL-6 protein in lung tissue is increased. The increase of IL-6 protein may be involved in the damage process.
2. Ambroxol Hydrochloride Injection has a certain effect on the protection of tracheal mucosa and lung tissue damage, and can reduce the expression of IL-6 protein in lung tissue.

【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R965

【参考文献】