石杉碱甲预处理对七氟醚麻醉新生大鼠学习记忆的影响

发布时间：2018-04-30 06:04

本文选题：石杉碱甲 + 预处理　；参考：《临床麻醉学杂志》2015年04期

【摘要】：目的探讨石杉碱甲预处理对七氟醚麻醉新生大鼠学习记忆的影响。方法 7日龄SD大鼠80只,雌雄不拘,体重15~20g,采用随机数字表法分为五组(n=16):对照组(C组)吸入30%的氧气6h,3%七氟醚麻醉2h组(S1组),预处理3%七氟醚麻醉2h组(AS1组),3%七氟醚麻醉6h组(S2组),预处理3%七氟醚麻醉6h组(AS2组),AS1组和AS2组麻醉前30min给予石杉碱甲0.2mg/kg腹腔注射。麻醉结束24h后,每组随机取8只大鼠断头取血,ELISA法检测血清S100β蛋白和神经元特异性烯醇化酶(NSE)浓度。五组余大鼠14日龄行Morris水迷宫实验。结果与C组比较,AS1组第1~6天逃避潜伏期、平台象限滞留时间、穿越平台次数无明显变化;AS2组第1~6天逃避潜伏期明显延长(P0.05),平台象限滞留时间、穿越平台次数无明显变化;余四组大鼠血清S100β及NSE浓度明显升高(P0.05)。与S1组比较,AS1组第1~6天逃避潜伏期无明显变化,平台象限滞留时间、穿越平台次数明显增加(P0.05),血清S100β和NSE浓度均明显降低(P0.05)。与S2组比较,AS2组第1~6天逃避潜伏期明显缩短、平台象限滞留时间、平台穿越次数明显增加(P0.05),血清S100β和NSE浓度均降低(P0.05)。结论石杉碱甲预处理能有效改善七氟醚麻醉新生大鼠学习记忆,对大脑神经细胞有一定的保护作用。
[Abstract]:Objective to investigate the effect of Huperzine A pretreatment on learning and memory in sevoflurane anesthetized neonatal rats. Methods Eighty SD rats of 7 days old, male and female, were selected. Body weight 15g / 20g, randomly divided into five groups: control group C) inhaling 30% oxygen 6 h, 3% sevoflurane anesthesia 2 h group, preconditioning 3% sevoflurane anesthesia group 3% sevoflurane anesthesia 6h group, preconditioning 3% sevoflurane anesthesia group, preconditioning 3% sevoflurane anaesthesia group, preconditioning 3% sevoflurane anesthesia group 30min was intraperitoneally injected with Huperzine A (0.2mg/kg) before anesthesia in AS2 group and AS2 group before anesthesia for 6 h. 24 hours after anesthesia, serum S100 尾 protein and neuron-specific enolase (NSEs) were measured by Elisa in 8 rats in each group. The Morris water labyrinth test was performed on the remaining 14 day old rats in the five groups. Results compared with group C, the escape latency of the 1st day, the platform quadrant, and the times of crossing the platform in AS1 group were not significantly changed. The escape latency of group AS2 on the 1st day of 6 days was significantly prolonged (P 0.05), and the time of platform quadrant retention was not significantly changed. Serum S 100 尾 and NSE concentration in the other four groups were significantly higher than those in the control group (P 0.05). Compared with S1 group, there was no significant change in escape latency in AS1 group on the 1st day, the time of staying in platform quadrant, the number of crossing platform increased significantly (P 0.05), and the concentrations of serum S100 尾 and NSE decreased significantly (P 0.05). Compared with S2 group, the escape latency of AS2 group on the 1st day was shortened, the retention time of platform quadrant, the number of platform crossing increased significantly (P 0.05), and the serum S100 尾 and NSE concentrations were decreased. Conclusion Huperzine A pretreatment can effectively improve the learning and memory of newborn rats anesthetized with sevoflurane and has a certain protective effect on brain nerve cells.
【作者单位】：新疆维吾尔自治区人民医院麻醉科;
【基金】：国家自然科学基金(81160016)
【分类号】：R614

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