榛蘑多糖对脂多糖诱导的急性肺损伤大鼠的保护作用

发布时间：2018-04-30 09:49

本文选题：榛蘑多糖 + 脂多糖　；参考：《延边大学》2017年硕士论文

【摘要】：目的:本研究以脂多糖建立急性肺损伤大鼠模型,用榛蘑多糖对其进行干预,探讨榛蘑多糖对急性肺损伤大鼠的影响及相关机制。方法:将35只健康SD雄性大鼠(清洁级)随机分配成5组,每组7只,分别设为正常组、模型组、阳性对照组、榛蘑多糖低、高(200mg/Kg、400mg/Kg)剂量组。用药组分别以低、高剂量榛蘑多糖灌胃10d,其他组以相同剂量的生理盐水灌胃。10d后,阳性对照组在脂多糖注射2h前予地塞米松(5mg/Kg)腹腔注射,2h后,除正常组外,所有大鼠均予腹腔注射脂多糖(20mg/Kg)诱导急性肺损伤,正常组给予腹腔注射等体积的PBS。脂多糖注射6h后,用20%乌拉坦麻醉后心脏采血处死所有大鼠,并解剖大鼠取肺脏。检测指标:(1)肺组织病理学观察;(2)采用ELISA法检测血浆中内皮素-l(ET-l)、血栓调节蛋白(TM)、白介素-6(IL-6)、肿瘤坏死因子-a(TNF-a)的含量;(3)用蛋白质免疫印迹试验(Western Blot)检测各组p-PI3K、p-Akt、NF-κB、p-NF-κB、Ik-B的表达,测定各条带灰度值。结果:1.肺组织病理示:脂多糖损伤后,肺泡结构破坏、肺泡间隔增厚、血管腔内出血、微血栓形成;与模型组相比,阳性对照组、低剂量组、高剂量组的肺泡结构较为完整,肺泡间隔变薄,血管腔出血及微血栓减少。2.ELISA法检测结果示:与正常组相比,.模型组TNF-a、IL-6、TM、ET-1的含量明显增多(P0.05);与模型组相比,榛蘑多糖高剂量组TNF-a、IL-6、TM、ET-1的含量明显减少(P0.05),榛蘑多糖低剂量组TM、ET-1的含量明显减少(P0.05)。3.Western Blotting检测结果显示:与正常组相比,模型组p-PI3K、p-Akt、NF-κB、p-NF-κB表达明显增多(P0.05),Ik-B表达明显减少(P0.05);和模型组相比,榛蘑低、高剂量组p-PI3K、p-Akt、NF-κ B、p-NF-κ B表达明显减少(P0.05),Ik-B表达明显增多(P0.05)。结论:1.榛蘑多糖对LPS诱导的急性肺损伤大鼠有保护作用。2.PI3K/Akt/NF-κ B信号通路可能参与了榛蘑多糖对LPS诱导的急性肺损伤大鼠的保护作用。
[Abstract]:Objective: to establish a rat model of acute lung injury with lipopolysaccharide, the effect of polysaccharide of Hazel mushroom on it was used to study the effect of Polysaccharide on acute lung injury in rats and its mechanism. Methods: 35 healthy SD male rats (clean grade) were randomly assigned into 5 groups, 7 rats in each group were set up as normal group, model group, positive control group, hazelnut more The group with low and high (200mg/Kg, 400mg/Kg) dose group. The group was fed with 10d with low and high dose of hazelnut polysaccharide, and the other groups were intraperitoneally injected with the same dose of normal saline for.10d. The positive control group was intraperitoneally injected with Yo Di Jose Misson (5mg/Kg) before lipopolysaccharide injection 2H. After 2h, all rats were injected with lipopolysaccharide (20mg/Kg) induced by intraperitoneal injection. After injection of PBS. lipopolysaccharide (6h) by intraperitoneal injection of equal volume of PBS. lipopolysaccharide injected into the normal group, all rats were killed with 20% urantan anesthesia and the lungs were dissected and the lungs were dissected. (1) pulmonary histopathological observation; (2) the ELISA method was used to detect endothelin -l (ET-l), thrombomodulin (TM), interleukins -6 (IL-6), and malignant tumor. The content of death factor -a (TNF-a); (3) the expression of p-PI3K, p-Akt, NF- kappa B, p-NF- kappa B, p-NF- kappa B, Ik-B were detected by protein immunoblotting test (Western Blot). Results: 1. lung tissue pathology showed alveolar structure destruction, alveolar septum thickening, intravascular hemorrhage and microthrombus formation after lipopolysaccharide injury; compared with model group The alveolar structure in the low dose group and the high dose group was more complete, the alveolar septum was thinner, the blood vessel hemorrhage and the microthrombus decreased by.2.ELISA method. Compared with the normal group, the content of TNF-a, IL-6, TM and ET-1 in the model group increased significantly (P0.05). Compared with the model group, the content of TNF-a, IL-6, TM and ET-1 in the high dose group of hazelnut polysaccharide was obvious. Decreased (P0.05), TM and ET-1 in the low dose group of hazelnut polysaccharide (P0.05).3.Western Blotting detection results showed that the expression of p-PI3K, p-Akt, NF- kappa B, p-NF- kappa B was significantly increased compared to the normal group, and the expression of p-NF- kappa B was significantly reduced. Significantly decreased (P0.05) and Ik-B expression increased significantly (P0.05). Conclusion: 1. the protective effect of polysaccharide of mushroom on acute lung injury induced by LPS in rats may be involved in the protective effect of.2.PI3K/Akt/NF- kappa B signaling pathway on the acute lung injury induced by the polysaccharides of the hazelnut mushroom.

【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R285.5

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