自体红骨髓一期移植治疗股骨粉碎型骨折

发布时间：2018-04-30 11:17

本文选题：股骨骨折 + 模型　；参考：《中国组织工程研究》2017年25期

【摘要】：背景:骨折愈合是否需要一期红骨髓移植,是否植入间充质干细胞?当股骨粉碎性骨折时进行闭式复位髓内钉内固定术,同期进行自体红骨髓一期移植是否可促进骨折处成骨的作用,尚处于研究阶段。目的:探讨采取闭式复位髓内钉内固定方法,一期治疗同时给予自体红骨髓移植对骨折愈合的影响。方法:选择健康新西兰兔20只,麻醉后以30 cm高度质量为5 kg重锤自由下落的做功条件制作兔股骨粉碎型骨折模型。给予2.0 mm直径克氏针髓内固定系统模仿股骨髓内钉内固定术对其进行固定。将兔股骨粉碎性骨折内固定造模成功的右侧股骨定义为骨折内固定组,将其左股骨定义为红骨髓移植组。红骨髓移植组将提取的1 mL自体红骨髓注射于骨折处。于造模后第14,28天分批处死10只兔,测量X射线片上骨折周围骨痂最厚处骨痂厚度;苏木精-伊红染色后光镜观察2组骨折处组织切片中软骨细胞数量及骨小梁相对面积。结果与结论:(1)骨折后14 d,骨折内固定组较红骨髓移植组骨折周围骨痂生成厚度明显减小(P0.01),差异有显著性意义;(2)骨折后14 d,红骨髓移植组骨折处软骨细胞生成数量大于骨折内固定组(P0.01),骨折愈合速度快于骨折内固定组;(3)骨折后28 d,骨折内固定组较红骨髓移植组骨折处骨小梁相对面积明显缩小(P0.01);(4)结果提示,红骨髓移植对于闭式复位髓内钉内固定修复兔股骨干粉碎型骨折的愈合具有促进作用。
[Abstract]:Background: does one stage red bone marrow transplantation and mesenchymal stem cells are needed for fracture healing? It is still in the research stage that closed reduction and intramedullary nail internal fixation and autologous red bone marrow transplantation at the same time can promote the osteogenesis of the fracture when the femoral comminuted fracture is treated with closed reduction and intramedullary nail fixation. Objective: to investigate the effect of closed reduction and intramedullary nail fixation and autologous red bone marrow transplantation on fracture healing. Methods: 20 healthy New Zealand rabbits were used to establish the femoral comminuted fracture model after anesthesia. A 2.0 mm diameter Kirschner needle fixation system was given to imitated femoral bone marrow nail fixation. The right femur was defined as the fracture internal fixation group and the left femur was defined as the red bone marrow transplantation group. In the red bone marrow transplantation group, 1 mL autologous red bone marrow was injected into the fracture. Ten rabbits were killed in batches on the 28th day after modeling, and the thickness of callus around fracture was measured, and the number of chondrocytes and the relative area of trabecular bone were observed by light microscope after hematoxylin-eosin staining. Results and conclusion at 14 days after fracture, the thickness of callus formation around the fracture in the internal fixation group was significantly reduced than that in the red bone marrow transplantation group, and the difference was significant (P 0.01). The number of chondrocytes in the fracture site of the red bone marrow transplantation group was significantly lower than that in the red bone marrow transplantation group at 14 days after the fracture. The fracture healing speed was faster than that in the fracture internal fixation group 28 days after the fracture. The relative area of trabecular bone in the fracture internal fixation group was significantly smaller than that in the red bone marrow transplantation group, and the results suggested that the bone trabecular area of the fracture group was significantly smaller than that of the red bone marrow transplantation group. Red bone marrow transplantation can promote the healing of femoral shaft comminuted fracture in rabbits with closed reduction and intramedullary nail fixation.
【作者单位】：内蒙古医科大学第二附属医院创伤骨科;内蒙古医科大学第二附属医院小儿骨科;
【基金】：内蒙古医科大学第二附属医院院基金(2015YJJ24)~~
【分类号】：R687.3

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