罗哌卡因所致惊厥对幼鼠学习记忆能力及海马区GFAP表达的影响

发布时间：2018-05-03 03:18

本文选题：罗哌卡因 + 惊厥　；参考：《石河子大学》2014年硕士论文

【摘要】：目的:研究罗哌卡因所导致的毒性惊厥对幼年SD鼠学习记忆能力及海马区星型胶质纤维酸性蛋白(GFAP)表达的影响。方法:选用60只4周龄SD幼鼠按照随机数字表法分为罗哌卡因组(以Racine惊厥分类中V级为标准制作模型)和生理盐水组(每组30只)。2组各取20只,并根据不同取材时间点又分为注射后24h、3d、7d、60d4个亚组（每组5只）。选取幼鼠海马组织，采用免疫组化法行海马区星型胶质纤维酸性蛋白GFAP的形态变化并计数;剩余10只于1~3d、5~7d、58~60d行进行水迷宫平台潜伏期测试。结果:水迷宫定向航行实验中罗哌卡因组在毒性惊厥后24h和第3d的逃避潜伏期均长于对照组（P＜0.05)，在第7d和第以后的60d，与对照组已无明显差别（P＞0.05）。罗哌卡因组的星型胶质细胞GFAP的表达明显弱于对照组，表现为胞体较小，突起细而短，，染色浅淡，且GFAP阳性细胞在数量上显著低于对照组（P＜0.05），尤以麻醉后第三天最为显著，细胞数呈先减少后增加的动态过程。结论:幼鼠单次罗哌卡因毒性惊厥对其学习记忆有一过性影响，推测与星型胶质纤维酸性蛋白功能有关。
[Abstract]:Objective: To study the effects of ropivacaine induced convulsion on learning and memory ability and astrocyte glial fibrillary acidic protein (GFAP) expression in hippocampus of young SD rats.
Methods: 60 4 week old SD young rats were divided into ropivacaine group according to random number table method (the standard making of V class in Racine convulsion classification) and.2 group of saline group (30 group of each group) each of 20 groups, and then divided into 24h, 3D, 7d, 60d4 subgroup (5 each group) after the different sampling time points. The hippocampus tissue of young rats was selected and immunized. The morphological changes of astrocyte glial fibrillary acidic protein GFAP in hippocampus were counted by histochemical method, and the remaining 10 in 1~3d, 5~7d and 58~60d were tested by water maze latency test.
Results: the escape latency of 24h and 3D in ropivacaine group was longer than that in the control group (P < 0.05). There was no significant difference between the control group and the control group (P > 0.05) at 7d and later 60d (P > 0.05). The expression of GFAP in ropivacaine group was significantly weaker than that of the control group, which showed that the cell body was smaller and the protuberance was fine. The number of GFAP positive cells was significantly lower than that of the control group (P < 0.05), especially in the third day after anesthesia, and the number of cells showed a dynamic process that decreased first and then increased.
Conclusion: single ropivacaine induced convulsion has a transient effect on learning and memory in young rats. It is speculated that it is related to the function of astroglial fibrillary acidic protein.

【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R614

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