芪芍颗粒治疗桥本甲状腺炎的实验研究

发布时间：2018-05-03 15:52

本文选题：桥本甲状腺炎 + 甲状腺球蛋白抗体　；参考：《湖北中医药大学》2017年硕士论文

【摘要】：目的:通过观察芪芍颗粒对实验性自身免疫性甲状腺炎(EAT)模型大鼠血清甲状腺球蛋白抗体(TGAb)、甲状腺过氧化物酶抗体(TPOAb)、白介素-6(IL-6)、白介素-12(IL-12)的影响,探讨芪芍颗粒防治桥本甲状腺炎(HT)的机理。材料与方法:采用SPF级SD雌性大鼠60只,周龄4-6周,体质量110±10g。具体方法如下:(1)第1周适应性喂养;(2)第2周将大鼠随机(采用随机数表法)分为正常对照组(15只)、造模组(45只),正常对照组给予蒸馏水,造模组给予浓度为0.64g/L的高碘水;(3)第4周开始给予造模组大鼠双足皮下多点注射由完全弗氏佐剂(CFA)充分乳化成油包水状的Tg(Tg:CFA=1:1),一共2次,中间间隔2天,作为初次免疫;(4)第5周开始给予大鼠后背皮下多点注射由弗氏不完全免疫佐剂(IFA)乳化的Tg(Tg:IFA=1:1),一共4次,中间间隔7天,作为加强免疫;第8周结束时成模,将成模后的大鼠随机(采用随机数表法)分为模型组(15只)、雷公藤多苷片组(15只)、芪芍颗粒组(15只);(5)第9周各组开始灌胃给药,雷公藤多苷片组每日给予雷公藤多苷片6.25mg/kg,芪芍颗粒组每日给予中药21.4g/kg,模型组及正常对照组每日给予等量的生理盐水;(6)灌胃治疗4周后腹腔麻醉,经腹主动脉取血离心并采用酶联免疫法(ELISA法)来检测各组大鼠血清中的IL-6、IL-12、TGAb、TPOAb的水平。结果:1.与正常对照组相比,模型组大鼠血清甲状腺抗体TPOAb、TGAb的水平明显升高,有显著性差异(p0.01);与模型组相比,芪芍颗粒组大鼠血清甲状腺抗体TPOAb、TGAb的水平明显降低,有显著性差异(p0.01);与雷公藤多苷片组相比,芪芍颗粒组大鼠血清甲状腺抗体TPOAb、TGAb的水平无明显变化,无显著性差异(p0.05)。2.与正常对照组相比,模型组大鼠血清IL-6、IL-12的水平明显升高,有显著性差异(p0.01);与模型组相比,芪芍颗粒组大鼠血清IL-6、IL-12的水平明显降低,有显著性差异(p0.01);与雷公藤多苷片组相比,芪芍颗粒组大鼠血清IL-6、IL-12的水平无明显变化,无显著性差异(p0.05)。结论:1.芪芍颗粒可显著降低EAT大鼠血清甲状腺抗体TGAb、TPOAb的水平。2.在降低EAT大鼠血清甲状腺抗体TGAb、TPOAb的水平上,芪芍颗粒与雷公藤多苷片相比,无显著性差异。3.芪芍颗粒能够显著降低EAT大鼠血清IL-6、IL-12的水平。4.在降低EAT大鼠血清IL-6、IL-12的水平上,芪芍颗粒与雷公多苷片相比,无显著性差异。5.芪芍颗粒与雷公藤多苷片治疗作用相当。
[Abstract]:Objective: To observe the effect of Qi Pai Granule on serum thyroglobulin antibody (TGAb), thyroid peroxidase antibody (TPOAb), interleukin -6 (IL-6) and -12 (IL-12) in experimental autoimmune thyroiditis (EAT) model rats, and to explore the mechanism of Qi Shao Granule on the prevention and control of Hashimoto's thyroiditis (HT). 60 rats of sex were 4-6 weeks of age, and the body mass of 110 + 10g. was as follows: (1) first weeks of adaptive feeding; (2) second weeks were randomly divided into normal control group (15) and model group (45), the normal control group was given distilled water and the model group was given the high iodine concentration of 0.64g/L; (3) the model rats began to be given to the model rats at week fourth. The multipoint injection of the bipedal subcutaneous injection was fully emulsified by the complete Freund's adjuvant (CFA) into a water like Tg (Tg:CFA=1:1), a total of 2 times, 2 days in the middle interval, as the primary immunization. (4) the rat's dorsal subcutaneous multipoint injection of Tg (Tg:IFA=1:1), which was emulsified by the Freund incomplete immune adjuvant (IFA), was given to the back of the rat's back, 4 times, for 7 days in the middle interval, as an enhanced immunization. At the end of the eighth week, the rat model was divided into model group (15 rats), the group of Tripterygium wilfordii (15) and Qi Shao granule group (15); (5) ninth weeks, each group began to give the medicine. The Tripterygium glycoside group was given the Tripterygium glycoside tablet 6.25mg/kg daily, and the Qi Pai granule group was given 21.4g/kg, the model group and the model group daily. The normal control group was given equal amount of normal saline daily; (6) intraperitoneal anesthesia after 4 weeks of gavage, blood centrifugation through abdominal aorta and enzyme linked immunoassay (ELISA) to detect the levels of IL-6, IL-12, TGAb and TPOAb in serum of each group. Results: 1. compared with the normal control group, the level of serum thyroid antibody TPOAb, TGAb level in the model group rats was compared with the normal control group. Significantly higher, there was significant difference (P0.01). Compared with the model group, the level of serum thyroid antibody TPOAb and TGAb in the rats of Qi Shao granule group decreased significantly (P0.01). Compared with the group of Tripterygium wilfordii polysaccharide, the level of serum thyroid antibody TPOAb, TGAb in the rats of Qi Shao granule group had no significant change, no significant difference (P0.05).2. and no significant difference. Compared with the normal control group, the level of serum IL-6 and IL-12 in the model group was significantly higher than that in the model group (P0.01). Compared with the model group, the serum IL-6, IL-12 level of the rats in the Qi Shao granule group decreased significantly (P0.01). Compared with the group of Tripterygium wilfordii polysaccharide, there was no significant change in the level of IL-6 and IL-12 in the serum of the rats of the Qi Pei granule group. There was no significant difference (P0.05). Conclusion: 1. Qi paehao granule can significantly reduce the serum thyroid antibody TGAb of EAT rats. The level.2. of TPOAb can reduce the serum thyroid antibody TGAb, TPOAb level of EAT rats. There is no significant difference between Qi Shao granule and Tripterygium wilfordii multi glycoside tablets, there is no significant difference between.3. Qi paehao granule and EAT rat serum IL-6, IL-12 water Flat.4. has no significant difference in the level of IL-6 and IL-12 in the serum of EAT rats. There is no significant difference between the Qi Pai granule and the Lei Gong poly glycoside tablets. The effect of.5. on the treatment is similar to that of Tripterygium wilfordii polysaccharide.

【学位授予单位】：湖北中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R285.5

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