低剂量全氟化碳不同时点干预急性肺损伤实验兔对肺组织病理学的影响

发布时间：2018-05-03 18:30

本文选题：全氟化碳 + 预处理　；参考：《广东医学》2015年17期

【摘要】：目的探讨低剂量全氟化碳(PFC)汽化吸入预处理和后处理对油酸型急性肺损伤(ALI)实验兔模型的肺组织病理的影响。方法新西兰大白兔18只,随机分成3组(n=6),PFC预处理组(Pre组)、PFC后处理组(Post组)和机械通气对照组(C组)。动物麻醉后气管插管行机械通气,Pre组和Post组分别在造模成功前后汽化吸入PFC 2 m L/(kg·h)60 min。C组不予PFC,机械通气60 min后,建立油酸型ALI模型。3组实验机械通气210 min。动物麻醉平稳30 min后定为T0,分别将机械通气(Post组和C组)60 min、PFC预处理(Pre组)60 min设定为T1,ALI造模后第30、60、90、150分钟设定为T2、T3、T4、T5时点,在各时点分别检测氧合指数(Pa O2/Fi O2)、平均动脉压和心率等。观察结束后处死动物,留取右肺上、中叶检测肺湿干重比,取右下肺组织分别在光镜下和大体观下行病理学检查和病理损伤评分。结果与Post组与C组各时点氧合指数相比,Pre组均显著升高(P0.05),Post组与C组相比差异无统计学意义(P0.05)。在肺湿干重比、右下肺病例损伤评分方面,Pre组显著低于Post组和C组(P0.05),Post组和C组之间则差异无统计学意义(P0.05)。病理学光镜下可见,Pre组以水肿渗出病变为主,伴小灶性出血,较C组和Post组有明显改善。Post组以炎症、坏死、渗出为主,与C组比较差异无统计学意义(P0.05)。3组实验在不同时点的血流动力学方面差异无统计学意义(P0.05)。结论在经气道吸入PFC 2 m L/(kg·h)的两种干预模式中,预处理能够显著改善ALI兔的氧合指数,减轻肺组织病理损伤。
[Abstract]:Objective to investigate the effects of preconditioning and post-treatment on lung histopathology of acute lung injury induced by oleic acid in rabbits with low dose perfluorocarbon perfluorocarbon perfluorocarbon (PFC) vaporization inhalation. Methods Eighteen New Zealand white rabbits were randomly divided into 3 groups: preconditioning group (pre group) and control group (group C). After anesthesia, endotracheal intubation was performed in the pre group and Post group respectively. The experimental mechanical ventilation of oleic acid ALI model group was established for 210 minutes after mechanical ventilation for 60 min before and after vaporization inhalation of PFC 2 m L/(kg hau 60 min.C before and after the successful establishment of the model. After 30 min of anesthesia, the animals were set as T0. The 60 min of preconditioning with PFC was set as the time point of T2T2T3T4T4 T5 after the model was made by T1A Ali. The oxygen index Pa O2/Fi O2, mean arterial pressure and heart rate were measured at each time point. After observation, the animals were sacrificed, left on the right lung, the lung wet / dry weight ratio was measured in the middle lobe, and the pathological examination and pathological injury score of the right lower lung tissue were performed under light microscope and gross observation, respectively. Results compared with Post group and C group, the oxygenation index of pre group was significantly higher than that of C group. There was no significant difference between P0.05 group and C group. Compared with Post group and C group, there was no significant difference in lung wet / dry weight ratio between right lower lung injury group and Post group (P 0.05) and C group (P 0.05). Pathological light microscope showed that edema and exudation were the main lesions in the pre group, accompanied by small focal hemorrhage. Compared with the C group and Post group, the inflammation, necrosis and exudation were significantly improved in the Pre group, which was mainly caused by inflammation, necrosis and exudation. There was no significant difference between group C and group C in the hemodynamics of group P0.053.There was no significant difference in hemodynamics at different time points. Conclusion in the two intervention models of inhaling PFC 2 m L/(kg h through the airway, pretreatment can significantly improve the oxygenation index of ALI rabbits and alleviate the pathological injury of lung tissue.
【作者单位】：广州医科大学附属第四医院麻醉科;广州医科大学附属第一医院麻醉科;
【基金】：广东社会发展领域科技计划(编号:2011B031800192) 广州市科技和信息化局应用基础研究专项项目(编号:2013J4100098) 广东省医学科研基金立项课题(编号:A2013300)
【分类号】：R614

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