猫杯状病毒不同分离株致病性的比较

发布时间：2018-05-04 12:42

本文选题：猫杯状病毒 + 分离株　；参考：《中国兽医学报》2015年07期

【摘要】：为确定猫杯状病毒(Feline calicivirus,FCV)SH、JL-1、JL-2分离株毒力强弱,将其分别人工接种6~11周龄健康非免疫家猫,分成接种组和对照组。接种前后分别测定各组猫体温、体质量,观察发病情况及临床症状,按照欧洲药典对症状进行计分。于感染后14d麻醉处死,制备病料组织切片,观察组织器官的病理变化。结果显示,家猫感染FCV后总发病率为100%,死亡率为66.7%;SH、JL-1、JL-2组临床症状平均得分分别为8、4、9,对照组得分为1。病理解剖观察发现,猫感染FCV后,以鼻、眼分泌物增加,口腔溃疡为特征,消化道病变较轻微,肺部出现不同程度的变性、出血,呈明显病理性"肉样变";病理组织学观察发现,肺脏有数量不等的肺泡上皮细胞和巨噬细胞脱落,伴随少量纤维蛋白渗出和弥漫性肺泡损伤。结果表明,FCV SH、JL-1、JL-2分离株均有一定的致病性,其中以JL-2株的毒力最强。
[Abstract]:In order to determine the virulence of Feline calicivirus FCVS JL-1 / JL-2 strain, we inoculated 61-week-old healthy non-immunized domestic cats by artificial inoculation and divided them into two groups: inoculation group and control group. Before and after inoculation, the body temperature and body mass of the cats were measured, the incidence and clinical symptoms were observed, and the symptoms were scored according to the European Pharmacopoeia. At the 14th day after infection, the rats were anesthetized and killed, the tissue sections were prepared and the pathological changes of tissues and organs were observed. The results showed that the total incidence of FCV infection in domestic cats was 100 and the mortality rate was 66.7. The average score of clinical symptoms in the JL-2 group was 84 / 9 and that in the control group was 1. Pathological observation showed that after infection with FCV, cats were characterized by increased nasal and eye secretion, oral ulcers, mild digestive tract lesions, varying degrees of degeneration and hemorrhage in the lungs. Histopathological observation showed that alveolar epithelial cells and macrophages were exfoliated, accompanied by a small amount of fibrin exudation and diffuse alveolar injury. The results showed that the isolates of JL-1 JL-2 of FCV had certain pathogenicity, and the virulence of JL-2 strain was the strongest.
【作者单位】：吉林农业大学动物科学技术学院;
【基金】：公益性行业(农业)科研专项(201303042)
【分类号】：S852.65

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