依托咪酯对神经胶质瘤细胞缝隙连接通讯的影响

发布时间：2018-05-07 04:02

本文选题：依托咪酯 + 神经胶质瘤　；参考：《中国癌症杂志》2015年07期

【摘要】：背景与目的:细胞缝隙连接(gap junction,GJ)通讯能增强放疗或化疗药物对肿瘤细胞的细胞毒性作用。以往研究发现部分麻醉药物能够改变GJ功能从而影响肿瘤细胞对放疗的敏感性。该研究拟观察依托咪酯对神经胶质瘤细胞由缝隙连接蛋白Cx43组成的缝隙连接功能的影响,为麻醉药对化疗敏感性影响的机制研究提供线索。方法:采用磺酰罗丹明B法观察依托咪酯对神经胶质瘤细胞的抑制作用;细胞接种荧光法观察依托咪酯对神经胶质瘤细胞GJ功能的影响。结果:0.1、0.5、1和5μmol/L依托咪酯在作用4 h时间内均对细胞生长无抑制作用,将不影响细胞缝隙的数量;取药物浓度接近血药浓度的依托咪酯作用细胞4 h,与对照组相比,0.1μmol/L依托咪酯作用细胞4 h后,神经胶质瘤细胞GJ通讯的荧光传递功能无明显变化;而0.5和1μmol/L依托咪酯作用细胞4 h后,神经胶质瘤细胞的荧光传递功能明显减弱。结论:依托咪酯能抑制神经胶质瘤细胞Cx43组成的GJ功能。
[Abstract]:Background & AIM: gap junctional GJC communication can enhance the cytotoxicity of radiotherapy or chemotherapy drugs to tumor cells. Previous studies have found that some anesthetic drugs can change the function of GJ and affect the sensitivity of tumor cells to radiotherapy. The aim of this study was to observe the effect of etomidate on the gap junction function of glioma cells composed of gap junction protein (Cx43), and to provide clues for the study of the mechanism of anesthetic effect on chemosensitivity. Methods: the inhibitory effect of etomidate on glioma cells and the effect of etomidate on the GJ function of glioma cells were observed by sulfonyl rhodamine B method. Results both 0. 1 and 0. 5 渭 mol/L etomidate had no inhibitory effect on cell growth within 4 h, and the number of cell gaps would not be affected. The cells treated with etomidate with drug concentration close to the concentration of plasma etomidate for 4 h, compared with the control group, the fluorescence transfer function of GJ communication in glioma cells did not change significantly after treated with etomidate 0.1 渭 mol/L for 4 h. After treated with etomidate for 4 h, the fluorescence transfer function of glioma cells was significantly decreased after 0. 5 渭 mol/L and 1 渭 mol/L etomidate treatment. Conclusion: etomidate can inhibit the GJ function of Cx43 in glioma cells.
【作者单位】：广州医科大学附属第二医院麻醉科;广州中医药大学附属第二医院麻醉科;
【基金】：国家自然科学基金项目资助(81401017) 广州市卫生局项目资助(20141A011083)
【分类号】：R739.41

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