增液润燥汤对干燥综合征NOD小鼠颌下腺中T、B淋巴细胞浸润及AQP5表达水平的影响

发布时间：2018-05-15 23:44

本文选题：增液润燥汤 + 干燥综合征　；参考：《中华中医药杂志》2017年08期

【摘要】：目的:观察增液润燥汤对干燥综合征(SS)NOD小鼠颌下腺中T、B淋巴细胞浸润情况以及AQP5表达水平的影响。方法:将40只8周龄NOD小鼠随机分为5组,即模型组,增液润燥汤低、中、高剂量组与羟氯喹组,同时选取8只同龄Balb/c小鼠作为正常组。增液润燥汤低、中、高剂量组分别给予10.5、21.0、42.0g/kg增液润燥汤灌胃;羟氯喹组给予60mg/kg羟氯喹灌胃;正常组与模型组给予同等剂量的去离子水灌胃。8周后,测量体质量后于麻醉下处死,摘取颌下腺、脾腺与胸腺称重并计算脏器指数;使用免疫组化法检测小鼠颌下腺中T、B淋巴细胞浸润量以及水通道蛋白5(AQP5)表达水平。结果:模型组小鼠颌下腺指数与正常组比较明显下降(P0.05),药物干预后小鼠颌下腺指数变化不明显。模型组小鼠脾脏和胸腺指数与正常比较变化不明显。免疫组化结果显示,正常组小鼠颌下腺中未见有T与B淋巴细胞的浸润,模型组小鼠颌下腺中可见有大量的T、B淋巴细胞浸润;干预8周后,增液润燥汤各剂量组小鼠颌下腺中T、B淋巴细胞浸润量与模型组比较皆明显下降(P0.05)。模型组小鼠AQP5的表达水平与正常组小鼠比较明显降低(P0.05);干预8周后,增液润燥汤各剂量组小鼠颌下腺中AQP5的表达水平皆明显上升(P0.05)。结论:NOD小鼠颌下腺中T和B淋巴细胞的浸润量明显增多、AQP5的表达明显减少;增液润燥汤能够有效抑制NOD小鼠颌下腺中T和B淋巴细胞浸润、促进AQP5的表达。
[Abstract]:Objective: to observe the effect of Zengye Runzao decoction on the infiltration of T cell B lymphocytes and the expression of AQP5 in the submandibular gland of Sjogren syndrome (SSS) mice. Methods: forty 8-week-old NOD mice were randomly divided into 5 groups: model group, Zengye Runzao decoction group and hydroxychloroquine group, and 8 Balb/c mice of the same age were selected as normal group. Zengye Runzao decoction was low, medium and high dose group was given 10.5 ~ 21.0g / kg Zeng-Runzao decoction respectively; hydroxychloroquine group was given 60mg/kg hydroxychloroquine orally; normal group and model group were given the same dose of deionized water for 8 weeks, and then killed under anesthesia. The submandibular gland, spleen gland and thymus gland were removed and the visceral index was calculated, and the infiltration of TnB lymphocytes and the expression of aquaporin 5 (aquaporin 5) in the submandibular gland of mice were detected by immunohistochemical method. Results: the index of submandibular gland in the model group was significantly lower than that in the normal group, but there was no significant change in the index of the submandibular gland in the model group after drug intervention. The indexes of spleen and thymus of model group were not significantly different from those of normal mice. The immunohistochemical results showed that there was no T and B lymphocyte infiltration in the submandibular gland of the normal mice, and a large number of T and B lymphocytes infiltrated in the submandibular gland of the model group. Compared with the model group, the infiltrating amount of Thunb lymphocytes in the submandibular gland of Zengye Runzao decoction group decreased significantly (P 0.05). The expression of AQP5 in the model group was significantly lower than that in the normal group, and after 8 weeks of intervention, the expression of AQP5 in the submandibular gland of the mice in the Zengye Runzao decoction group was significantly increased (P 0.05). Conclusion the infiltrating amount of T and B lymphocytes in the submandibular gland of NOD mice was obviously increased and the expression of AQP5 was decreased obviously, and Zengye Runzao decoction could effectively inhibit the infiltration of T and B lymphocytes and promote the expression of AQP5 in the submandibular gland of NOD mice.
【作者单位】：北京中医药大学;北京中医药大学东方医院;中国医学科学院北京协和医学院北京协和医院;
【基金】：北京市自然科学基金资助项目(No.7132198)~~
【分类号】：R285.5

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