小剂量地塞米松对生长期小鼠骨代谢的影响

发布时间：2018-05-16 00:12

  本文选题：地塞米松 + 生长期　； 参考：《中南大学》2014年硕士论文

【摘要】：目的：观察小剂量地塞米松对生长期小鼠骨代谢的影响。 方法：24只4周龄的雌性C57B1/6J小鼠随机分两组,每组各12只：地塞米松组(DEX组),右侧股四头肌肌注1mg/kg体重地塞米松；对照组。每周称量小鼠体重并记录。所有小鼠干预4周后予以腹腔注射巴比妥麻醉,颈椎脱臼法处死小鼠后立即摘眼球取血,分离血清,酶联免疫吸附法(enzyme linked immunosorbent assay, ELISA)检测Ⅰ型前胶原N端前肽(PINP)和骨Ⅰ型胶原交联C端顶端肽(CTX-Ⅰ)蛋白的表达水平；取一侧胫骨行显微CT扫描分析,扫描完成后进行三维重建,用显微CT自带的MicroView2.0+ABA软件对松质骨表观骨密度、组织骨密度、骨体积分数、骨面积分数、骨小梁厚度、骨小梁间隔、骨小梁数量、结构模型指数等指标进行分析。并建立各组松质骨二维图像；免疫组化(immunohistochemistry IHC)方法检测胫骨干骺端骨保护素(osteoprotegerin OPG)、核因子-κB受体活化因子配体(receptor activator for nuclear factor-κB ligand, RANKL)蛋白表达；抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase TRAP)染色方法检测破骨细胞。 结果：与对照组相比,DEX组小鼠胫骨干骺端表观骨密度及骨体积分数明显增高(p0.05),而血清PINP及β-CTX水平明显下降(p0.05)。地塞米松促进小鼠胫骨干骺端OPG蛋白表达,而对RANKL表达无明显影响,导致OPG/RANKL比例显著升高；与对照组相比,DEX组小鼠胫骨干骺端破骨细胞数量明显减少。 结论：本研究发现,小剂量的地塞米松可能对生长期小鼠骨量增长有促进作用,其机制可能在于通过调节OPG/RANKL的比例,抑制骨吸收,降低骨转换率。
[Abstract]:Objective: to observe the effect of low dose dexamethasone on bone metabolism in growing mice. Methods Twenty four four-week-old female C57B1/6J mice were randomly divided into two groups: dexamethasone group (n = 12), dexamethasone (1mg/kg) in right quadriceps femoris muscle group, and control group (n = 12). The mice were weighed weekly and recorded. After 4 weeks of intervention, all the mice were given intraperitoneal injection of barbitole anesthesia, and the mice were killed by cervical dislocated method. The blood was taken from the eyeball and the serum was separated immediately after the mice were killed. Enzyme linked immunosorbent assay (linked immunosorbent assay, ELISA) was used to detect the expression of PINP- 鈪，

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