小半夏汤对顺铂诱发大鼠脑干孤束核Fos蛋白表达的影响

发布时间：2018-05-17 13:30

本文选题：顺铂 + Fos蛋白　；参考：《时珍国医国药》2017年11期

【摘要】：目的观察小半夏汤对顺铂化疗大鼠脑干孤束核Fos蛋白表达的影响。方法雄性大鼠随机分为正常对照组(10ml·kg~(-1)蒸馏水i.g.)、顺铂模型组(0.1ml·kg~(-1)蒸馏水i.g.)、昂丹司琼治疗组(昂丹司琼2.6mg·kg~(-1),i.g.)、阿瑞吡坦治疗组(阿瑞吡坦11.1mg·kg~(-1),i.g.)、小半夏汤治疗组(小半夏汤1.6g·kg~(-1),i.g.),单笼饲养。模型组和各治疗组6 mg·kg~(-1)顺铂i.p.造模。从造模前3 d开始,各组动物每天按照上述剂量分别灌胃相应药物2次(间隔12 h)直至处死。注射顺铂后24 h、72 h深度麻醉大鼠,心脏灌注,取脑,冰冻切片,免疫组化技术观察各组大鼠孤束核内Fos阳性细胞数。结果与空白对照组比较,注射顺铂后24 h、72孤束核的Fos阳性细胞数均显著增加(P0.001),造模后24h时间点的增加程度大于72 h。小半夏汤治疗组与顺铂模型组比较,两个时间点Fos阳性细胞数均显著降低(P0.001);与空白对照组比较,小半夏汤治疗组Fos阳性细胞数有增加趋势,但无统计学意义(P0.05);与两种阳性药组分别进行比较,小半夏汤治疗组Fos阳性细胞数没有显著性差异(P0.05)。结论顺铂能够使大鼠孤束核Fos蛋白表达显著增加,小半夏汤可以抑制顺铂导致的孤束核Fos蛋白表达增加。
[Abstract]:Objective to observe the effect of Xiao Banxia decoction on the expression of Fos protein in brain stem solitary tract nucleus of cisplatin chemotherapy rats. Methods male rats were randomly divided into three groups: normal control group (n = 10 ml) and cisplatin model group (n = 0.1 ml 路kg ~ (-1) in distilled water (i.g.), Ondansetron treatment group (2.6mg / kg ~ (-1) i.g., Arelpidem treatment group (11.1mg kg ~ (-1), small Banxia decoction group (small Banxia decoction 1.6 g kg 路kg ~ (-1) i.g.), single cage feeding, and the control group (n = 10) were randomly divided into three groups: the control group (n = 10), the control group (n = 10); Model group and treatment group (6 mg 路kg ~ (-1) cisplatin i.p. Make a model. From the 3 days before the model was made, the animals in each group were given the corresponding drugs twice a day (12 hours interval) according to the above dosage until they were killed. The number of Fos positive cells in the nucleus tractus solitarii of rats in each group was observed by immunohistochemical technique. The rats were anesthetized for 72 hours after injection of cisplatin, the heart was perfused, brain was taken, frozen sections were taken and the number of Fos positive cells was observed by immunohistochemistry. Results compared with the control group, the number of Fos positive cells in the nucleus tractus solitarii at 24 h after injection of cisplatin increased significantly (P 0.001), and the increase at 24 h after injection was more than 72 h. Compared with cisplatin model group, the number of Fos positive cells in Xiaobanxia decoction group was significantly lower than that in cisplatin model group, and the number of Fos positive cells in Xiaobanxia decoction treatment group was increased compared with the blank control group. However, there was no significant difference in the number of Fos positive cells between the two groups, and there was no significant difference in the number of Fos positive cells between the two groups. Conclusion cisplatin can significantly increase the expression of Fos protein in rat nucleus of solitary tract, and Xiaobanxiatang can inhibit the expression of Fos protein in nucleus of solitary tract induced by cisplatin.
【作者单位】：山东中医药大学;
【基金】：国家自然科学基金面上项目(81373828)
【分类号】：R285.5

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