小鼠机械性肺损伤肺组织中microRNA表达差异的研究

发布时间：2018-05-26 16:43

本文选题：microRNA + 急性肺损伤　；参考：《中国医学前沿杂志(电子版)》2017年02期

【摘要】：目的研究小鼠机械通气所致急性肺损伤(acute lung injury,ALI)/急性呼吸窘迫综合征(acute respiratory distress syndrome,ARDS)肺组织中microRNAs的差异表达及其意义。方法将20只小鼠按照随机数表法分为模型组(10只)与对照组(10只)。模型组小鼠麻醉下气管插管后予肌松药,并予10 ml/kg潮气量行机械通气,构建(ALI/ARDS)模型。对照组小鼠麻醉下气管插管后不予肌松药,且不行机械通气,使其自主呼吸。24小时后将所有小鼠处死,测量左肺组织湿重/干重比值,HE染色观察右中肺病理变化,取肺泡灌洗液行肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白介素-β(interleukin-β,IL-β)水平检测。应用microRNA芯片技术检测两组小鼠肺组织中microRNA表达,对存在差异的microRNA进行靶基因预测,并对靶基因行信号通路分析。结果模型组小鼠呼吸系统症状明显,肺组织湿重/干重比值明显高于对照组(P0.01),与ALI/ARDS病理结果相符。模型组小鼠TNF-α、IL-1β表达水平显著高于对照组(P0.05)。芯片结果显示模型组与对照组小鼠间存在34个明显差异表达的microRNA,上调19个,下调15个。结论机械性ALI/ARDS病理生理可能与microRNA调控的炎症相关信号通路等有关。
[Abstract]:Objective to study the differential expression and significance of microRNAs in acute lung injuryus / acute respiratory distress syndrome (ARDS) induced by mechanical ventilation in mice. Methods 20 mice were divided into model group (n = 10) and control group (n = 10). Model group mice were given muscle relaxant and 10 ml/kg tidal volume after tracheal intubation and mechanical ventilation to construct ALI / ARDS model. In the control group, no muscle relaxant was given after tracheal intubation and no mechanical ventilation. All the mice were killed after spontaneous respiration for 24 hours. The left lung tissue wet weight / dry weight ratio and HE staining were measured to observe the pathological changes of the right middle lung. The level of tumor necrosis factor- 伪 (TNF- 伪) and interleukin- 尾 (IL- 尾) in alveolar lavage fluid were measured. MicroRNA microarray technique was used to detect the expression of microRNA in lung tissues of the two groups of mice. The target gene was predicted by differential microRNA and the signal pathway of the target gene was analyzed. Results the respiratory system symptoms and the ratio of wet weight to dry weight of lung tissue in the model group were significantly higher than those in the control group (P 0.01), which were consistent with the pathological results of ALI/ARDS. The expression level of TNF- 伪 -1 尾 in the model group was significantly higher than that in the control group (P 0.05). The results of microarray showed that there were 34 differentially expressed microRNAs between the model group and the control group, 19 upregulated and 15 down-regulated. Conclusion the pathophysiology of mechanical ALI/ARDS may be related to the inflammatory signaling pathway regulated by microRNA.
【作者单位】：首都医科大学附属北京胸科医院北京市结核病胸部肿瘤研究所;
【分类号】：R563.8

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