隔药饼灸对功能性胃肠病大鼠CRF、NT的影响

发布时间：2018-05-27 22:02

  本文选题：功能性胃肠病 + 隔药饼灸　； 参考：《湖南中医药大学》2014年硕士论文

【摘要】：目的：以功能性胃肠病(FIGDs)大鼠为研究对象,观察隔药饼灸对FIGDs大鼠的治疗作用；并运用ELISA法检测实验大鼠促肾上腺激素释放因子(CRF)与神经降压素(NT)的血清含量,以了解隔药饼灸对功能性胃肠病大鼠CRF、NT的影响,进而探讨隔药饼灸是否通过调节FIGDs大鼠的CRF、NT水平而起到治疗作用,以期为隔药饼灸治疗FIGDs的临床应用提供一定的实验依据。 方法：60只SD大鼠随机分为5组：空白组(A组)、模型组(B组)、隔药饼灸组(C组)、杏仁核注射组(D组)、假处理组(E组)；各组先进行适应性饲喂。正式实验开始后除A组正常饲喂,其余各组均接受FIGDs造模；采用慢性束缚应激+过度疲劳+饮食失节的造模方法并在此基础上加夹尾、摇晃以造成FIGDs模型。于每日开始造模前30min,C组捆绑固定后进行隔药饼灸,取Ⅰ：肝俞、脾俞、足三里；Ⅱ：章门、期门、足三里两组穴位交替施灸；B、D、E组则只捆绑不施灸,造模连续进行21日；第22日D组进行双侧杏仁核微量注射CNQX溶液,E组则接受双侧杏仁核微量注射生理盐水；自第22日起所有实验大鼠均自由吃食饮水4日；所有实验大鼠于第26天麻醉后取材并将标本送检。标本用ELISA法测CRH、NT的血清含量；光镜下观察胃肠组织的形态。分别于造模前、初次束缚后、正式实验的第14、第21、第26日观察各组大鼠一般情况；分别于造模前、造模结束、治疗后测量各组大鼠体重、食量、尿D-木糖排泄率的变化并记录。 结果：造模结束后,接受造模各组大鼠一般状态变差,腹泻便溏,体重、食量、尿D-木糖排泄率均低于空白组且差异均有统计学意义(P0.01)。治疗后,接受造模各组各项指标较造模结束时均有所回升,C、D组高于模型组且差异有统计学意义(P0.01),E组与模型组比较差异无统计学意义。经光镜观察胃肠组织：空白组：结肠组织结构完整,细胞状态良好；隔药饼灸组与CNQX组：结肠壁结构完整,细胞排列与形态良好,未见炎症表现；而模型组与假处理组均有炎症表现。C、D组CRF、NT水平高于B组,差异有统计学意义(P0.01)。 结论： 1.隔药饼灸和CNQX注射均通过上调CRF、NT水平起到维护机体应激调节功能,增加抗应激能力及抗炎症、保护细胞的作用,能改善实验大鼠的应激消耗状态,对功能性胃肠病起到治疗作用。 2.隔药饼灸和杏仁核微量注射CNQX对CRF、NT的调节作用可能均与杏仁核AMPA受体相关。
[Abstract]:Objective: to observe the therapeutic effect of herbal cake moxibustion on FIGDs rats in rats with functional gastrointestinal disease (FIGDs), and to detect the serum levels of adrenocorticotropic hormone releasing factor (CRFF) and neurotensin (NTN) in experimental rats by ELISA method. In order to understand the effect of herbal cake moxibustion on CRFN NT in rats with functional gastroenteropathy, and to explore whether the moxibustion of medicine-separated cake could play a therapeutic role by regulating the level of CRFN in FIGDs rats, the purpose of this study was to provide some experimental evidence for the clinical application of drug separated cake moxibustion in the treatment of FIGDs. Methods Sixty Sprague-Dawley rats were randomly divided into five groups: control group (group A), model group (group B), moxibustion group (group C), amygdala injection group (group D) and sham treatment group (group E). At the beginning of the formal experiment, all the other groups were fed with FIGDs except group A, and the FIGDs model was made by using the method of chronic restraint stress and excessive fatigue diet disjointing and adding tail clip to make FIGDs model. The moxibustion of medicine-separated cake was carried out 30 minutes before and 30 minutes before the model was made in group C, and the moxibustion was performed continuously for 21 days in group 鈪，

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