柴胡疏肝散对功能性消化不良大鼠胃排空的促进作用及机制

发布时间：2018-05-28 12:20

本文选题：功能性消化不良 + 柴胡疏肝散　；参考：《山东医药》2017年37期

【摘要】：目的观察柴胡疏肝散对功能性消化不良(FD)大鼠胃排空的促进作用,并探讨其作用机制。方法将24只健康成年SD大鼠随机均分为正常组、模型组、柴胡疏肝散组与多潘力酮组。除正常组外,其余各组均采用夹尾刺激法制备FD模型。各组均于造模第1天给予药物干预,柴胡疏肝散组、多潘立酮组分别给予0.63 g/mL柴胡疏肝散水煎剂1.5 mL、0.6 mg/mL多潘立酮水溶液1.5 mL灌胃,正常组、模型组均给予生理盐水1.5 mL灌胃,2次/d,共干预4周。末次给药后24 h,各组予以营养性半固体糊(1.5 mL/100 g体质量)灌胃,30 min后麻醉开腹,剪取完整胃,根据全胃重、空胃重计算胃排空率。用HE染色法观察胃窦部黏膜组织病理变化,用免疫组化法检测胃窦部黏膜组织中蛋白激酶R样内质网激酶(PERK)和磷酸化真核翻译起始因子2α(p-eIF2α)蛋白表达。结果与正常组比较,模型组大鼠胃排空率低(P0.05);与模型组比较,柴胡疏肝散组、多潘立酮组胃排空率高(P均0.05)。模型组、柴胡疏肝散组和多潘立酮组胃窦组织可见少量淋巴细胞及中性粒细胞浸润。与正常组比较,模型组大鼠胃窦组织内PERK、p-eIF2α蛋白表达高(P均0.05);与模型组比较,柴胡疏肝散组、多潘立酮组胃窦组织内PERK、p-eIF2α蛋白表达低(P均0.05)。结论柴胡疏肝散可能通过调控内质网应激信号通路PERK/eIF2α促进FD大鼠胃排空。
[Abstract]:Objective to observe the promoting effect of Chaihu Shugan Powder on gastric emptying in rats with functional dyspepsia (FDD) and to explore its mechanism. Methods 24 healthy adult SD rats were randomly divided into normal group, model group, chaihu Shugan powder group and doxalidone group. Except the normal group, the FD model was made by clip-tail stimulation. Each group was given drug intervention on the first day of model making. The group of Chaihu Shugan Powder and the group of domperidone were given 0.63 g/mL of decoction of Shugan Powder of Bupleurum Bupleurum and 1.5 mL of water solution of domperidone for 0.6 mg/mL, respectively, and the normal group were given oral administration of 1.5 mL of domperidone solution, respectively. The model group were given normal saline 1.5 mL intragastric perfusion twice a day for 4 weeks. 24 hours after the last administration, each group was treated with nutritional semisolid paste (1.5 mL/100 g body mass) for 30 min. After anesthesia, the complete stomach was cut and the gastric emptying rate was calculated according to the weight of the whole stomach and the weight of the empty stomach. The pathological changes of gastric antrum mucosa were observed by HE staining. The expression of protein kinase R-like endoplasmic reticulum kinase (PERK) and phosphorylated eukaryotic translation initiation factor (2 伪) -eIF2 伪 in antral mucosa were detected by immunohistochemical method. Results compared with the normal group, the gastric emptying rate in the model group was lower than that in the model group, and the gastric emptying rate in the Chaihu Shugan powder group and doperidone group was 0.05. In model group, Chaihu Shugan Powder group and domperidone group, a small amount of lymphocytes and neutrophils were infiltrated in gastric antrum. Compared with the normal group, the expression of PERKN p-eIF2 伪 protein in the antral tissue of the model group was higher than that in the model group, and that in the chaihu Shugan powder group and the doperidone group was lower than that in the model group, and the expression of PERKO p-eIF2 伪 protein in the antral tissue of the model group was lower than that in the control group (P 0.05). Conclusion Chaihu Shugan Powder can promote gastric emptying in FD rats by regulating endoplasmic reticulum stress signaling pathway PERK/eIF2 伪.
【作者单位】：广西医科大学第一附属医院;
【基金】：国家自然科学基金资助项目(81560763)
【分类号】：R285.5

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