依托咪酯对大鼠空间学习记忆能力及海马CA1区LTD的影响研究

发布时间：2018-06-02 15:19

本文选题：依托咪酯 + Morris水迷宫　；参考：《新疆医科大学》2014年硕士论文

【摘要】：目的：通过Morris水迷宫研究依托咪酯对于大鼠空间学习记忆能力的影响及采用全细胞膜片钳并结合药理学方法,以长时程抑制(Long-term depression,LTD)为指标,研究依托咪酯对大鼠空间学习记忆能力及海马CA1区长时程抑制的影响。方法：给予大鼠依托咪酯(5mg/kg)腹腔注射作为实验组,给予脂肪乳剂腹腔注射作为溶剂对照组,给予生理盐水腹腔注射作为空白对照组,然后应用Morris水迷宫评价依托咪酯对大鼠空间学习记忆的影响。之后应用全细胞电压钳技术,观察30μl依托咪酯(相当于5μmol/L)脂肪乳剂对大鼠海马CA1区神经元LTD的影响以及依托咪酯影响LTD的可能机制。结果：给予大鼠依托咪酯腹腔注射可以影响大鼠的空间学习记忆能力。依托咪酯可诱导LTD成功,且LTD表达增强；LTD的易化作用能被GABAA受体竞争性拮抗剂荷包牡丹碱阻断。结论：研究表明多次给大鼠腹腔注射依托咪酯重复实施麻醉或镇静可以影响其空间学习记忆能力,且依托咪酯麻醉可以增强LTD的表达,其机制可能影响大鼠空间学习记忆能力。
[Abstract]:Objective: to study the effect of etomidate on spatial learning and memory ability of rats by Morris water maze and to use whole-cell patch clamp and pharmacological method to study the long term expression of etomidate. To study the effects of etomidate on spatial learning and memory ability and long term inhibition of hippocampal CA1 in rats. Methods: the rats were given 5 mg / kg etomidate intraperitoneally as experimental group, fat emulsion as solvent control group and saline as blank control group. Then Morris water maze was used to evaluate the effect of etomidate on spatial learning and memory in rats. Then the effects of 30 渭 l etomidate (equivalent to 5 渭 mol / L) fat emulsion on LTD in rat hippocampal CA1 neurons and the possible mechanism of etomidate affecting LTD were observed by whole-cell voltage clamp technique. Results: intraperitoneal injection of etomidate could affect the ability of spatial learning and memory in rats. Etomidate could induce the success of LTD, and the enhancement of LTD expression could be blocked by bicuculline, a competitive antagonist of GABAA receptor. Conclusion: repeated intraperitoneal injection of etomidate or sedation may affect the ability of spatial learning and memory in rats, and etomidate anesthesia can enhance the expression of LTD, and its mechanism may affect the ability of spatial learning and memory in rats.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R614

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