针刺对脑卒中后抑郁模型大鼠行为学的影响

发布时间：2018-06-04 10:19

本文选题：针刺 + 脑卒中后抑郁　；参考：《安徽中医药大学》2014年硕士论文

【摘要】：目的：观察分析针刺百会、风府、神门、太冲穴对脑卒中后抑郁（Post-strokedepression,PSD）模型大鼠行为学、神经功能缺损以及体重的影响，为针灸治疗脑卒中后抑郁提供科学依据。方法：采用大脑中动脉线拴阻塞法模型（MCAO），大鼠在清醒后第7天，相继给予慢性温和不可预测应激（CUMS）处理，，制备脑卒中伴抑郁的复合模型。运用Longa评分方法，进行5分制评分，以评定脑卒中大鼠神经功能的缺损与恢复；运用糖水消耗实验评定PSD大鼠兴趣及快感的变化；运用敞箱实验评定PSD大鼠对事物的好奇程度以及自发运动的变化等抑郁行为的改变。结果：在MACO模型建立2小时大鼠清醒后进行神经功能缺损评分，各组造模大鼠的Longa评分均在1-3分，24小时后大鼠神经功能缺损基本恢复正常，Longa评分均为0分，说明大鼠在手术麻醉清醒后出现了病灶对侧肢体轻度功能障碍，出现明显神经功能缺损，空白组与模型组大鼠体重以及行为学各项评估结果进行对比，模型组大鼠体重及行为学评估结果均有明显降低（P＜0.05），提示PSD模型成功。治疗后针刺组与西药组大鼠体重及行为学评估结果显示，两治疗组均疗效显著（P＜0.05），但两组差异不明显（P＞0.05）。结论：采用大脑中动脉线拴阻塞法模型复合慢性不可预知性应激抑郁症模型可以成功制备脑卒中后抑郁的动物模型。针刺百会、风府、神门、太冲穴治疗脑卒中后抑郁有效，可以增加体重、糖水消耗量以及提高垂直运动次数和水平运动次数，且较盐酸氟西汀治疗脑卒中后抑郁，疗效无明显差异。通过本实验研究证实针刺百会、风府、神门、太冲穴是防治脑卒中后抑郁大鼠的有效治疗手段，具有研究和进一步开发的价值。
[Abstract]:Objective: to observe and analyze the effects of acupuncture at Baihui, Fengfu, Shenmen and Taicong points on the behavior, nerve function defect and body weight of rats with post-stroke depression and provide scientific basis for acupuncture and moxibustion treatment of post-stroke depression. Methods: the middle cerebral artery occlusion model was used. Rats were treated with chronic mild unpredictable stress (CUMS) on the 7th day after waking to establish a complex model of stroke with depression. The Longa scoring method was used to evaluate the neurological deficit and recovery of stroke rats, and the changes of interest and pleasure of PSD rats were evaluated by sugar water consumption test. Open box experiment was used to evaluate the degree of curiosity and the changes of spontaneous motion in PSD rats. Results: after 2 hours of MACO model was established, the neurological impairment scores were evaluated in rats. The Longa scores of the rats in each group were 0 after 1-3 minutes and 24 hours after the neural function defect was basically restored to normal. The results showed that the rats had mild dysfunction of contralateral limbs and obvious neurological impairment after operation. The results of body weight and behavioral evaluation were compared between the blank group and the model group. The results of body weight and behavioral evaluation in the model group were significantly lower than that in the control group (P < 0.05), indicating that the PSD model was successful. After treatment, the results of body weight and behavior evaluation in the acupuncture group and the western medicine group showed that the curative effect of the two groups was significant (P < 0.05), but the difference between the two groups was not significant (P > 0.05). Conclusion: the model of middle cerebral artery occlusion combined with chronic unpredictable stress depression can be successfully used to establish the animal model of post-stroke depression. Acupuncture at Baihui, Fengfu, Shenmen and Taicong points is effective in treating post-stroke depression. It can increase body weight, consumption of sugar and water, increase the number of vertical and horizontal movements, and is more effective than fluoxetine hydrochloride in treating post-stroke depression. There was no significant difference in curative effect. This experiment proved that acupuncture at Baihui, Fengfu, Shenmen and Taichong is an effective therapeutic method for prevention and treatment of post-stroke depression in rats, which has the value of research and further development.
【学位授予单位】：安徽中医药大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R245

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