清肺解毒饮对肺炎链球菌肺炎、肺炎克雷伯菌肺炎两种老龄肺炎大鼠肺组织TNF-α、IFN-γ、IL-6及IL-10含量变化的

发布时间：2018-06-06 07:58

本文选题：清肺解毒饮 + 肺炎链球菌　；参考：《安徽中医药大学》2014年硕士论文

【摘要】：目的：通过建立肺炎链球菌与肺炎克雷伯菌两种老龄大鼠的肺炎模型，运用清肺解毒饮治疗两种老龄肺炎大鼠后，观察其肺组织内炎症因子TNF-α，IFN-γ，IL-6，IL-10含量的变化，比较给药前后肺组织内炎症因子指标的变化，给清肺解毒饮治疗两种细菌性肺炎提供实验依据。方法:选用SD老龄大鼠20-22月龄，体重400g±50g，将140只（SPF级）SD老龄雄性大鼠随机分为7组，分别为正常组、肺炎链球菌老龄大鼠模型组、肺炎克雷伯菌老龄大鼠模型组及两种肺炎模型治疗的清肺解毒饮高、低剂量组(分别简称肺链高剂量组、肺链低剂量组和肺克高剂量组、肺克低剂量组)。每组均20只，适应性喂养一周，参照Yershov等方法，分别将肺炎链球菌、肺炎克雷伯菌气管内注入。将腹腔注射10%水合氯醛0.35mL/100g使大鼠麻醉，并经上中切牙点悬吊，用一接注射器的钝圆针头经口插入气管，分别滴入40μL约含有107CFU处于对数生长期的肺炎链球菌、肺炎克雷伯菌混悬液(在滴入时堵住鼻孔)，使接种物入肺，急性肺炎很快诱导成功。按上述方法造模后灌胃给药，每组给药剂量均根据大鼠体重按成人一日剂量折算[按公式大鼠等效剂量(mg/kg)=折算系数×成人剂量(mg/kg)]计算出大鼠等效剂量：中药：8.19g/kg，中药高、低剂量组分别给予中药16.4、4.10g/(kg·d)灌胃,正常组和两种老龄大鼠肺炎模型组分别灌以生理盐水。连续给药14天，然后将大鼠处死。以检查大鼠胸片、实验室检测及免疫组化的改变。运用安徽中医药大学第一附属医院呼吸科临床常用方剂清肺解毒饮治疗后，观察清肺解毒饮对两种肺炎大鼠肺组织内TNF-α，IFN-γ，，IL-6，IL-10含量的变化，比较给药前后肺炎指标的改变。结果：ELISA分析结果显示，肺炎链球菌肺炎大鼠经治疗14天后的高剂量组肺组织内含有的TNF-α、IFN-γ、IL-6、IL-10明显低于模型组，有统计学意义。肺炎克雷伯菌肺炎大鼠经治疗14天后的高剂量组肺组织内含有的TNF-α、IFN-γ、IL-6、IL-10明显低于模型组，有统计学意义。两种肺炎大鼠的低剂量治疗组与其相对应的模型组之间无统计学意义。结论：清肺解毒饮在有效剂量下对肺炎链球菌及肺炎克雷伯菌两种肺炎大鼠有治疗作用，对相关细胞因子有调节作用。
[Abstract]:Objective: to establish the pneumonia model of two kinds of aged rats of Streptococcus pneumoniae and Klebsiella pneumoniae, and to observe the changes of IL-10 content of TNF- 伪 IFN- 纬 IL-6 in lung tissue of two aged rats treated with Qingfei jiedu decoction. The changes of inflammatory factors in lung tissue before and after administration were compared to provide experimental evidence for Qingfei jiedu decoction in the treatment of two kinds of bacterial pneumonia. Methods: 140 aged SD rats aged 20 to 22 months and weighing 400 g 卤50 g were randomly divided into 7 groups: normal group and streptococcus pneumoniae model group. The Qingfei jiedu decoction treated by Klebsiella pneumoniae in aged rat model group and two kinds of pneumonia models were high and low dose groups (respectively referred to as lung chain high dose group, lung chain low dose group and lung chain high dose group, lung Ke low dose group). Twenty rats in each group were fed adaptively for one week. According to Yershov method, streptococcus pneumoniae and Klebsiella pneumoniae were injected into trachea respectively. The rats were anesthetized by intraperitoneal injection of 10% chloral hydrate (0.35mL/100g), suspended from the upper incisor, and inserted into the trachea with a blunt round needle attached to a syringe. 40 渭 L of Streptococcus pneumoniae containing 107CFU in logarithmic growth phase was dripped into the trachea, respectively. Klebsiella pneumoniae suspension (clogging the nostril at the time of dripping, allowing the inoculum to enter the lung, the acute pneumonia is quickly induced. According to the above method, the dosage of each group was converted according to the adult daily dose according to the body weight of rats [according to the formula, the equivalent dose of rats was calculated by the formula: the equivalent dose of Chinese medicine was 8.19 g / kg 路kg ~ (-1), and the dose of Chinese herbal medicine was high, according to the formula, the equivalent dose was 8.19 g 路kg ~ (-1) 路kg ~ (-1), and the dose of each group was 8.19 g / kg 路kg ~ (-1). The low dose group was given 16.4 ~ 4.10 g / kg / d, and the normal group and two kinds of aged rat pneumonia model group were given normal saline respectively. Rats were killed after 14 days of continuous administration. The changes of chest radiographs, laboratory examination and immunohistochemistry in rats were examined. After treatment with Qingfei jiedu decoction, a commonly used prescription in Department of Respiratory, the first affiliated Hospital of Anhui University of traditional Chinese Medicine, the changes of TNF- 伪 IFN- 纬 -IFN- 纬 IL-6 IL-10 in lung tissue of two kinds of pneumonia rats were observed, and the changes of pneumonia indexes before and after administration were compared. Results the percentage of TNF- 伪 IFN- 纬 IL-6 IL-10 in lung tissue of the high dose group was significantly lower than that of the model group after 14 days of treatment, the results showed that the IL-10 level in the lung tissue of the rats treated with streptococcus pneumoniae was significantly lower than that in the model group. The TNF- 伪 IFN- 纬 -IFN- 纬 IL-6 IL-10 in lung tissue of high dose group after 14 days of treatment with Klebsiella pneumoniae was significantly lower than that of model group. There was no significant difference between the low-dose treatment group and the corresponding model group. Conclusion: Qingfei jiedu decoction has therapeutic effect on Streptococcus pneumoniae and Klebsiella pneumoniae pneumonia rats under the effective dosage, and it can regulate related cytokines.
【学位授予单位】：安徽中医药大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R259

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