液体复苏方式对休克大鼠肠屏障功能及炎性反应的影响

发布时间：2018-06-07 10:06

本文选题：失血性休克 + 液体复苏　；参考：《宁夏医学杂志》2016年12期

【摘要】：目的观察不同液体复苏方式对失血性休克大鼠肠屏障功能的影响,并探讨其机制。方法将健康雄性SD大鼠48只随机均分为4组,麻醉后右侧股动、静脉置管。3组休克大鼠在随后的60 min急救期接受输血输液等救治后拔除导管、缝合后回笼。液体复苏后24 h各组大鼠取标本备检测,分光光度法测小肠及血浆二胺氧化酶(DAO)活性、硝酸还原酶法测定肠组织一氧化氮(NO)含量、双抗夹心酶联免疫法(ELISA)测肠组织肿瘤坏死因子-α(TNF-α)含量。结果复苏后24 h,B组血浆DAO活性与C组相比显著较低(P0.05),而肠组织DAO显著增高(P0.05)。各休克组大鼠细菌移位发生率、肠组织TNF-α生成量、NO含量较对照组均有不同程度升高,其中B组大鼠肠组织TNF-α生成量、NO含量均低于C组(P0.05)。结论对失血性休克大鼠采用限制性液体复苏有利于减少肠组织TNF-α及NO生成,减轻肠黏膜屏障损伤,从而降低细菌移位的发生。
[Abstract]:Objective to investigate the effects of different fluid resuscitation methods on intestinal barrier function in hemorrhagic shock rats. Methods 48 male Sprague-Dawley rats were randomly divided into 4 groups. The rats in the right femoral artery and vein catheterization group were treated with transfusion and transfusion for 60 min after anesthesia. 24 hours after fluid resuscitation, the contents of nitric oxide (no) in intestinal tissues were determined by nitrate reductase method. The activity of diamine oxidase (DAO) in small intestine and plasma was measured by spectrophotometry. The content of TNF- 伪 in intestinal tissue was measured by Elisa with double antibody sandwich enzyme linked immunosorbent assay (Elisa). Results 24 hours after resuscitation, plasma DAO activity in group B was significantly lower than that in group C (P 0.05), while DAO in intestinal tissue was significantly higher than that in group C (P 0.05). The incidence of bacterial translocation and the content of TNF- 伪 and no in intestinal tissue of rats in each shock group were higher than those in control group, and the content of TNF- 伪 in group B was lower than that in group C (P 0.05). Conclusion restrictive fluid resuscitation in hemorrhagic shock rats can reduce the production of TNF- 伪 and no in intestinal tissue and reduce the injury of intestinal mucosal barrier, thus reducing the occurrence of bacterial translocation.
【作者单位】：宁夏医科大学总医院麻醉科;
【基金】：宁夏自然科学基金资助项目(NZ14280)
【分类号】：R459.7

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