乳清粉对断奶实验兔肠道微生物区系和益生菌的影响

发布时间：2018-06-07 21:11

本文选题：实验兔 + 乳清粉　；参考：《中国比较医学杂志》2015年08期

【摘要】：目的利用分子生物学技术研究饲粮中添加乳清粉对断奶早期实验兔肠道微生物区系和益生菌的影响。方法采用单因子实验设计,选择40日龄断奶实验兔,随机分为4组(每组12只),分别饲以乳清粉添加水平为0%、2%、5%和10%的饲粮。饲喂30 d后,每组随机取8只兔,麻醉处死,取盲肠内容物提取细菌总DNA,首先利用PCR-DGGE技术分析兔盲肠微生物区系多样性,进而应用实时荧光定量PCR(SYBR Green I)法定量检测盲肠细菌中双歧杆菌和乳酸杆菌含量。结果 (1)实验兔盲肠细菌DGGE各分析参数随乳清粉添加量的提高逐渐上升,添加不同水平乳清粉均可显著增加DGGE条带数(P0.05,P0.01)。2%和5%乳清粉添加水平组的DGGE条带数和香农指数均极显著(P0.01)高于未添加组,但以上2项指标在乳清粉各添加水平间差异无显著性(P0.05)。DGGE均匀度指数在各实验组间均无差异显著性(P0.05)。(2)饲粮中添加乳清粉可提高实验兔盲肠内容物中乳酸杆菌和双歧杆菌数量,其中各乳清粉处理组(2%,5%和10%)的乳酸杆菌数量均显著高于0%添加组(P0.05),10%乳清粉添加组的双歧杆菌数量显著高于未添加组(P0.05),但与其他2个乳清粉添加组无差异显著性(P0.05)。结论 (1)饲粮中添加乳清粉可显著提高实验兔盲肠细菌菌群的多样性。(2)在实验兔饲粮中添加乳清粉可有效增加肠道益生菌数量。
[Abstract]:Objective to study the effects of whey powder added to the diet on the intestinal microflora and probiotics in the early weaning rabbits. Methods a single factor experimental design was used to select 40 day old weanling experimental rabbits and randomly divided into 4 groups (12 rats in each group). The diets were fed with whey powder addition level of 0%, 2%, 5% and 10%, respectively. After feeding 30 d, 8 rabbits in each group were taken randomly, and the total DNA of bacteria was extracted from the cecum. First, the diversity of the cecum microbial flora was analyzed by PCR-DGGE technique, and the content of bifidobacteria and Lactobacillus in the cecum bacteria was detected by real time fluorescence quantitative PCR (SYBR Green I). Results (1) the analysis parameters of the experimental rabbit cecum bacteria DGGE were analyzed. As the addition of whey powder increased, the number of DGGE bands (P0.05, P0.01).2% and 5% whey powder adding level group were significantly increased by adding different levels of whey powder. The number of DGGE bands and Shannon index were significantly higher than those in the non added group, but the above 2 indexes had no significant difference (P0.05).DGGE between the addition levels of the whey powder. There was no significant difference in the evenness index between the experimental groups (P0.05). (2) the addition of whey powder in the diet could increase the number of Lactobacillus and Bifidobacterium in the experimental rabbit cecum content, and the number of Lactobacillus in each whey powder treatment group (2%, 5% and 10%) was significantly higher than that of the 0% addition group (P0.05), and the number of Bifidobacterium in the 10% whey powder addition group showed a significant number. It was higher than that of the unadded group (P0.05), but there was no difference with the other 2 whey powder groups (P0.05). Conclusion (1) the addition of whey powder in the diet can significantly increase the diversity of the experimental rabbit cecum bacterial flora. (2) the addition of whey powder in the experimental rabbit diet can effectively increase the number of intestinal probiotics.
【作者单位】：浙江中医药大学动物实验研究中心;浙江省医学科学院;
【基金】：浙江省科技厅资助项目基金(编号:2008F0003) 浙江中医药大学比较医学创新团队基金(编号:XTD201301)
【分类号】：R151

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