高效液相色谱法测定小鼠血清中磺胺间甲氧嘧啶及其N~4-乙酰化代谢物含量

发布时间：2018-06-10 01:24

  本文选题：磺胺间甲氧嘧啶 + N-乙酰化磺胺间甲氧嘧啶　； 参考：《中国兽药杂志》2015年02期

【摘要】：磺胺间甲氧嘧啶(SMM)于ICR小鼠哺乳期暴露后,测定其血清中SMM和N4-乙酰化磺胺间甲氧嘧啶(ACSMM)含量。小鼠哺乳期以0、10、50、200 mg/(kg·d)灌胃给药直至21 d,于第22天麻醉,摘眼球取血。血清样品经乙腈沉淀蛋白,超声辅助提取,采用反相C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈:水(含0.2%冰乙酸)为流动相,流速为1 mL/min,柱温35℃,高效液相色谱耦合二极管阵列检测器对目标物检测,SMM和ACSMM检测波长分别为273 nm和267 nm,外标法峰面积定量。SMM和ACSMM血清加标回收率为85.8%~110.2%,精密度(RSD%)均小于8.3%,在0.05~2.5μg/mL和5~30μg/mL(适用母鼠体内SMM)范围内与峰面积呈良好的线性关系,r20.990;最低检出限分别为0.023和0.042μg/mL。本法灵敏、快速、准确,可用于血清中SMM和ACSMM的测定。
[Abstract]:The levels of SMM and N4-acetylsulfamethoxifen (ACSMMMMM) in serum of ICR mice during lactation were determined. During lactation, the mice were given intragastric administration of the drug for 21 days, and were anesthetized on the 22nd day to get blood from their eyeballs. Serum samples were precipitated by acetonitrile and extracted by ultrasound. The chromatographic column was C18250 mm 脳 4.6 mm ~ (5 渭 m). Acetonitrile: water (containing 0.2% glacial acetic acid) was used as mobile phase, flow rate was 1 mL / min, column temperature was 35 鈩，

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