商陆皂苷甲对水负荷大鼠肾脏AQP2及AQP4表达的影响

发布时间：2018-06-10 02:57

本文选题：商陆皂苷甲 + AQP　；参考：《中药材》2015年08期

【摘要】：目的:观察商陆皂苷甲对水负荷大鼠肾脏水通道蛋白2(AQP2)及水通道蛋白4(AQP4)表达的调节作用,研究商陆皂苷甲利尿作用机制。方法:将50只雄性SD大鼠随机分为空白对照组、氢氯噻嗪组及商陆皂苷甲高、中、低剂量组,腹腔注射给药,氢氯噻嗪组给予氢氯噻嗪0.4 mg/kg,商陆皂苷甲高、中、低剂量组分别给5.2、2.6、1.3 mg/kg商陆皂苷甲,收集并测量给药后6 h内的尿量;水负荷实验结束后将大鼠麻醉处死,运用免疫组化及Real Time-PCR技术检测大鼠肾脏AQP2、AQP4蛋白及mRNA表达的变化。结果:与空白对照组比较,商陆皂苷甲高剂量组大鼠尿量显著增多,肾脏AQP2、AQP4蛋白及mRNA的表达显著下调(P0.05);商陆皂苷甲中、低剂量组尿量及肾脏AQP2、AQP4蛋白及mRNA表达差异无统计学意义。结论:商陆皂苷甲通过下调大鼠肾脏AQP2、AQP4蛋白及mRNA表达,使水分重吸收降低,可能是其利尿作用的机制之一。
[Abstract]:Aim: to investigate the effects of pokeweed saponins A on the expression of aquaporin 2AQP2and aquaporin 4AQP4 in the kidney of water-loaded rats, and to study the mechanism of poke-saponin A diuretic action. Methods: fifty male Sprague-Dawley rats were randomly divided into control group, hydrochlorothiazide group and pokeweed saponins A high, middle and low dose groups, intraperitoneal injection of hydrochlorothiazide 0.4 mg / kg, pokeweed saponin A high, medium. The rats in the low dose group were given 5.2 ~ 2.6A 1.3 mg/kg pokeweed saponin A, and the urine volume was collected and measured within 6 hours after administration, and the rats were killed under anesthesia after the water loading experiment. The changes of AQP2AQP4 protein and mRNA expression in the rat kidney were detected by immunohistochemistry and Real Time-PCR technique. Results: compared with the blank control group, the urine volume of high dose group of pokeweed saponin A increased significantly, and the expression of AQP2AQP4 protein and mRNA in kidney decreased significantly (P 0.05), but there was no significant difference in urine volume and renal AQP2AQP4 protein and mRNA expression in low dose group. Conclusion: pokeweed saponin A can decrease water reabsorption by down-regulating the expression of AQP2AQP4 protein and mRNA in rat kidney, which may be one of the mechanisms of its diuretic effect.
【作者单位】：山西中医学院制药与食品工程学院;湖北中医药大学药学院;
【基金】：国家自然科学基金面上项目(81173555)
【分类号】：R285.5

【相似文献】